The Power of Pairing Adaptive Deconvolution with Computational Clearing

Kohli, Vikram; Marr, James M.; Schlicker, Oliver; Felts, Levi

February 16, 2021

The Power of Pairing Adaptive Deconvolution with Computational Clearing

Technical Brief

Mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI. Sample is a FluoCells™ prepared slide #3 from Thermo Fisher Scientific, Waltham, MA, USA. Images courtesy of Dr. Reyna Martinez – De Luna, Upstate Medical University, Department of Ophthalmology. The_Power_of_Pairing_Adaptive_Deconvolution_teaser.jpg

Deconvolution is a computational method used to restore the image of the object that is corrupted by the point spread function (PSF) along with sources of noise. In this technical brief, learn how the deconvolution algorithm offered by Leica Microsystems allows you to overcome losses in image resolution and contrast in widefield (WF) fluorescence microscopy due to the wave nature of light and the diffraction of light by optical elements. Explore the methods of user-controlled or automated deconvolution to see and resolve more structural detail.

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