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FusionOptics – Combines high resolution and depth of field for ideal 3D optical Images
A study carried out jointly by Leica Microsystems and the Institute of Neuroinformatics at the University of Zurich and Swiss Federal Institute of Technology provided the basis for an innovation in…Read article
Beware of "Empty" Magnification
This article explains how to avoid the phenomen of "empty magnification" in microscopy.Read article
Digital Cameras
Manufacturers digital cameras race to outdo each other with ever-increasing numbers of megapixels. The world record for professional medium format digital cameras has now surpassed 60 megapixels per…Read article
A Guide to Super-Resolution Fluorescence Microscopy
For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass…Read article
Pinhole Geometry: Four Corners are Perfect
Square and hexagonal pinholes provide identical image signal levels, if the geometries are compared in a sensible manner. The amount of light passing the pinhole depends on the area of that aperture,…Read article
Video Talk by Jeff Lichtman: Resolution in Microscopy – Wave Optics and the Diffraction Limit
Light has properties of particles and waves. Understanding the wave nature of light is essential to understanding the workings of a microscope. This lecture describes Huygens Wavelets,…Read article
FusionOptics in Neurosurgery and Ophthalmology – for a Larger 3D Area in Focus
Neurosurgeons and ophthalmologists deal with delicate structures, deep or narow cavities and tiny structures with vitally important functions. A clear, three-dimensional view on the surgical field is…Read article
Video Talk by Jeff Lichtman: Point Spread Function
An infinitesimally small point appears in the microscope as a spot with a certain size, blurred in the z-direction and with concentric rings around it. This "point spread function" reveals many of the…Read article
Multi-Images Deconvolution Improves Signal-to-Noise Ratio on Gated Stimulated Emission Depletion Microscopy
Time-gated detection, namely, only collecting the fluorescence photons after a time-delay from the excitation events, reduces complexity, cost, and illumination intensity of a stimulated emission…Read article
Pathways to Optical STED Microscopy
STED nanoscopy has evolved to a highly versatile tool for the observation of the living cell, more and more finding its way into state-of-the-art optical imaging facilities in biomedical research…Read article
Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent
Super-resolution imaging deep inside tissues has been challenging, as it is extremely sensitive to light scattering and spherical aberrations. Here, we report an optimized optical clearing agent for…Read article
Quantifying the Resolution of a Leica SR GSD 3D Localization Microscopy System with 2D and 3D Nanorulers
DNA origami based nanorulers produced by GATTAquant are common standards to test the achievable spatial resolution of super-resolution microscopes. Recently the nanorulers were used to test the…Read article
Mirror-Enhanced Super-Resolution Microscopy
Axial excitation confinement beyond the diffraction limit is crucial to the development of next-generation, super-resolution microscopy. STimulated Emission Depletion (STED) nanoscopy offers lateral…Read article
Measuring the 3D STED-PSF with a new Type of Fluorescent Beads
A new type of fluorescent bead is presented by GATTAquant. These beads, called GATTA-Beads, are characterized by a small diameter (23 nm), high intensity and size uniformity. In combination with…Read article
Practical Guide for Excellent GSDIM Super-Resolution Images
Do you know that most protists and bacteria lack in one feature that each of our body cell has? Our cells are touch and communicate with one another. They send and receive a variety of signals that…Read article
Visualization of Membrane Dynamics with Millisecond Temporal Resolution
Application Note for Leica EM ICE, Leica EM AFS2 - Electrical stimulation of neurons combined with high-pressure freezing allows physiological activation of synaptic activity and precise control over…Read article
Microscope Resolution: Concepts, Factors and Calculation
In microscopy, the term ‘resolution’ is used to describe the ability of a microscope to distinguish detail. In other words, this is the minimum distance at which two distinct points of a specimen can…Read article
Super-Resolution Optical Microscopy of Lipid Plasma Membrane Dynamics
Plasma membrane dynamics are an important ruler of cellular activity, particularly through the interaction and diffusion dynamics of membrane-embedded proteins and lipids. FCS (fluorescence…Read article
Video Talk by Kurt Thorn: The Abbe Diffraction Experiment
This lecture describes the famous experiments of Ernst Abbe which showed how diffraction of light by a specimen (and interference with the illuminating light) gives rise to an image and how collection…Read article
Collecting Light: The Importance of Numerical Aperture in Microscopy
Numerical aperture (abbreviated as ‘NA’) is an important consideration when trying to distinguish detail in a specimen viewed down the microscope. NA is a number without units and is related to the…Read article
Immersion Objectives: Using Oil, Glycerol, or Water to Overcome some of the Limits of Resolution
To examine specimens at high magnifications using the microscope, there are a number of factors which need to be taken into consideration. These include resolution, numerical aperture (NA), the…Read article
Koehler Illumination: A Brief History and a Practical Set Up in Five Easy Steps
The technique of Koehler Illumination is one of the most important and fundamental techniques in achieving optimum imaging in any given light microscope set-up. Although it should be routinely used as…Read article
Eyepieces, Objectives and Optical Aberrations
For most microscope applications, there are generally only two sets of optics which are adjusted by the user, namely, the objectives and the eyepieces. Of course, this is assuming that the microscope…Read article
What Does 30,000:1 Magnification Really Mean?
One important criterion concerning the performance of an optical microscope is magnification. This report will offer digital microscopy users helpful guidelines to determine the useful range of…Read article
Evaluating Axon Regeneration After Brain or Spine Trauma of Mice
Damaged nerve regeneration was investigated using mouse spinal cord sections treated with compounds that counter axon growth inhibitor (AGI) proteins. The sections were screened to find active and…Read article
Key Factors to Consider When Selecting a Stereo Microscope
Stereo microscopes are often nicknamed the “workhorse” of the lab or production site. Users spend many hours looking through the eyepieces inspecting, observing, documenting, or dissecting samples.…Read article
The Power of Pairing Adaptive Deconvolution with Computational Clearing
Deconvolution is a computational method used to restore the image of the object that is corrupted by the point spread function (PSF) along with sources of noise. In this technical brief, learn how the…Read article