Cryo Electron Microscopy
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How to Target Fluorescent Structures in 3D for Cryo-FIB Milling
This article describes the major steps of the cryo-electron tomography workflow including super-resolution cryo-confocal microscopy. We describe how subcellular structures can be precisely located in…Read article
Precise 3D Targeting for EM Imaging - Access What Matters
Find out how the seamless cryo-electron tomography workflow Coral Cryo uses confocal super resolution to target your structure of interest more precisely.Read article
How to Successfully Perform Live-cell CLEM
The Leica Nano workflow provides a streamlined live-cell CLEM solution for getting insight bout structural changes of cellular components over time. Besides the technical handling described in the…Read article
How to Successfully Implement Coral Nano
The live-cell CLEM workflow allows you to capture dynamic information related to a relevant biological process as it happens and put these observations into their ultrastructural context. The Leica…Read article
Advancing Cellular Ultrastructure Research
Freeze-fracture and freeze-etching are useful tools for studying flexible membrane-associated structures such as tight junctions or the enteric glycocalyx. Freeze-fracture and etching are two…Read article
The Cryo-CLEM Journey
This article describes the Cryo-CLEM technology and the benefits it can provide for scientists. Additionally, some scientific publications are highlighted. Recent developments in cryo electron…Read article
How to Keep Your Samples Under Physiological Conditions
Leica Microsystems’ Nano workflow combines dynamic data with the best possible sample fixation (EM ICE 70) by high pressure freezing. However, good sample preservation won’t help if your cells are…Read article
Leica Nano Workflow Webinar
With the Leica Nano Workflow, searching for the needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the…Read article
Download EM Workflow Solutions Booklet
This publication is a compilation of appropriate workflows for the most frequently used sample preparation methods, like Correlative Methodologies, Optogenetics & Electro-Physiology, Surface Analysis,…Read article
Exploring the Structure and Life Cycle of Viruses
The SARS-CoV-2 outbreak started in late December 2019 and has since reached a global pandemic, leading to a worldwide battle against COVID-19. The ever-evolving electron microscopy methods offer a…Read article
Fast, high-quality vitrification with the EM ICE High Pressure Freezer
The EM ICE High Pressure Freezer was developed with a unique freezing principle and uses only a single pressurization and cooling liquid: liquified nitrogen (LN2). This design enables three major…Read article
Targeting Active Recycling Nuclear Pore Complexes using Cryo Confocal Microscopy
In this article, how cryo light microscopy and, in particular cryo confocal microscopy, is used to improve the reliability of cryo EM workflows is described. The quality of the EM grids and samples is…Read article
Investigating Synapses in Brain Slices with Enhanced Functional Electron Microscopy
A fundamental question of neuroscience is: what is the relationship between structural and functional properties of synapses? Over the last few decades, electrophysiology has shed light on synaptic…Read article
Advancing Cell Biology with Cryo-Correlative Microscopy
Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with…Read article
High-pressure freezing: Revealing functional mechanisms of synaptic transmission
Learn more about applying optogenetic stimulation in the EM ICE and how this technology has the potential to reveal structural and functional mechanisms of synaptic transmission. Get a detailed…Read article
Workflows and Instrumentation for Cryo-electron Microscopy
Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron…Read article
Coronavirus Replication in Host Cells
In this study, a molecular pore complex, which spans both membranes of SARS-CoV-2/COVID-19 virus-induced cytosolic vesicles and likely plays a role in replication, was visualized.Read article
Crystal clear cryo light-microscopy images
This article describes how computational clearing of cryo light microscopy images improves the identification of cellular targets for cryo electron-microscopy.Read article