Immunofluorescence
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Tumor MHC Expression and Intralesional IL2 Response in Melanoma
Genomics profiling and Cell DIVE multiplex imaging allows researchers to understand the immune cell phenotypes that most strongly predict response to IL2 immunotherapy in melanoma patients suffering…Read article
Multiplex Imaging Reveals Survival Markers after Cancer Care
Colorectal cancer is a high incidence and high mortality cancer. Currently, postoperative chemotherapy benefits only a minority of patients, and thus, new tools are necessary to screen patients and…Read article
Hyperplex Cancer Tissue Analysis at Single Cell Level with Cell DIVE
The ability to study how lymphoma cell heterogeneity is influenced by the cells’ response to their microenvironment, especially at the mutational, transcriptomic, and protein levels. Protein…Read article
How to Prepare Your Specimen for Immunofluorescence Microscopy
Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures. IF preparations can be analyzed by various microscopy techniques (e.g. CLSM,…Read article
Fluorescent Dyes
A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescent agent. This can be a fluorescent protein – for example GFP –…Read article
Designing your Research Study with Multiplexed IF Imaging
Multiplexed tissue analysis is a powerful technique that allows comparisons of cell-type locations and cell-type interactions within a single fixed tissue sample. It is common for researchers to ask…Read article
Be Confident in your Results with Cell DIVE Validated Antibodies
The Cell DIVE System includes a carefully curated list of hundreds of commercially available antibodies validated to offer optimal specificity and sensitivity in multiplexed imaging. That validation…Read article
How Can Immunofluorescence Aid Virology Research?
Modern virology research has become as crucial now as ever before due to the global COVID-19 pandemic. There are many powerful technologies and assays that virologists can apply to their research into…Read article
Upright Fluorescence Microscopy for Virus Replication Studies
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in late 2019 and spread over the world quickly. Due to its dramatic impact, researchers are interested in the nature of the…Read article
Infection of the novel coronavirus SARS-CoV-2 can be blocked by a human monoclonal antibody
Vaccines and targeted therapeutics for treatment of the respiratory disease COVID-19, caused by the coronavirus SARS-CoV-2, are currently lacking. Based on the results of this study, it was reported…Read article
Multiphoton imaging bolsters findings on in vitro differentiation of pluripotent stem cell-derived podocyte
The renal corpuscle of the kidney comprises a glomerular vasculature embraced by podocytes and supported by mesangial myofibroblasts. Their combined actions are essential for the formation of an…Read article
The Guide to STED Sample Preparation
This guide is intended to help users optimize sample preparation for stimulated emission depletion (STED) nanoscopy, specifically when using the TCS SP8 STED 3X nanoscope from Leica Microsystems. It…Read article
Chronic Inflammation Under the Microscope
In the course of chronic inflammation certain body areas are recurrently inflamed. This goes along with many human diseases. With the help of widefield light microscopy, the underlying processes can…Read article
Visualizing Tropoelastin in a Long-Term Human Elastic Fibre Cell Culture Model
Elastin is an essential protein found in a variety of tissues where resilience and flexibility are needed, such as the skin and the heart. When aiming to engineer suitable implants, elastic fibres are…Read article
BABB Clearing and Imaging for High Resolution Confocal Microscopy
Multipohoton microscopy experiment using Leica TCS SP8 MP and Leica 20x/0.95 NA BABB immersion objective. Understanding kidney microanatomy is key to detecting and identifying early events in kidney…Read article
Sample Preparation for GSDIM Localization Microscopy – Protocols and Tips
The widefield super-resolution technique GSDIM (Ground State Depletion followed by individual molecule return) is a localization microscopy technique that is capable of resolving details as small as…Read article
Protein Trafficking in Cereal Seeds
Seeds accumulate proteins and starch which will be broken down and mobilized upon germination. Among seeds, cereals constitute an example of a highly specialized storage tissue that constitutes up to…Read article
Subcellular Localization of AKT and Tubulin using Super-Resolution Microscopy
Stimulated Emission Depletion microscopy, or STED nanoscopy, is a technique that uses the non-linear de-excitation of fluorescent dyes to overcome the resolution limit imposed by diffraction…Read article