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Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection
Organotypic cultures of neuronal tissue were first introduced by Hogue in 1947 and have constituted a major breakthrough in the field of neuroscience. Since then, the technique was developed further…Read article
How to Study Protein Recruitment to DNA Lesions by a Combination of UV Laser and White Light Laser
Understanding how DNA lesions are optimally repaired is of functional significance, especially from the view of genome karyotype stability.Read article
Dry Ultrathin Sectioning Combined With High Pressure Freezing
We have used cultured UMR106-01 osteoblastic cells to investigate the process of bone mineralization. UMR106-01 cells as well as primary calvarial bone cells assembly spherical extracellular…Read article
Detailed Morphological Characterisation of Hendra Virus Infection of Different Cell Types Using Super-Resolution and Conventional Imaging
Hendra virus (HeV) is a pleomorphic virus belonging to the Paramyxovirus family. Our long-term aim is to understand the process of assembly of HeV virions. As a first step, we sought to determine the…Read articleHow to do a Proper Cell Culture Quick Check
In order to successfully work with mammalian cell lines, they must be grown under controlled conditions and require their own specific growth medium. In addition, to guarantee consistency their growth…Read article
Visualizing Tropoelastin in a Long-Term Human Elastic Fibre Cell Culture Model
Elastin is an essential protein found in a variety of tissues where resilience and flexibility are needed, such as the skin and the heart. When aiming to engineer suitable implants, elastic fibres are…Read article
Introduction to Mammalian Cell Culture
Mammalian cell culture is one of the basic pillars of life sciences. Without the ability to grow cells in the lab, the fast progress in disciplines like cell biology, immunology, or cancer research…Read article
Confluency Check with PAULA Cell Imager
Many cell-based experiments require cells in a certain state. This can include their morphology, fluorescent protein expression, and the confluency of the cell layer. Researchers check their cells…Read article
How can PAULA help streamline the workflow for my wound healing assay?
The wound healing assay, often used as a synonym for scratch assay or migration assay, is an important tool for the evaluation and measurement of cell migration, whether single cells or cell…Read article
Infection of the novel coronavirus SARS-CoV-2 can be blocked by a human monoclonal antibody
Vaccines and targeted therapeutics for treatment of the respiratory disease COVID-19, caused by the coronavirus SARS-CoV-2, are currently lacking. Based on the results of this study, it was reported…Read article
Upright Fluorescence Microscopy for Virus Replication Studies
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in late 2019 and spread over the world quickly. Due to its dramatic impact, researchers are interested in the nature of the…Read article
Coronavirus Replication in Host Cells
In this study, a molecular pore complex, which spans both membranes of SARS-CoV-2/COVID-19 virus-induced cytosolic vesicles and likely plays a role in replication, was visualized.Read article
A Quality Metric for the Systematic Evaluation of Clearing Protocols
3D multicellular spheroids are of interest for studying tumor behavior and evaluating the response of pharmacologically active agents, because they mimic the in vivo tumor environment better than…Read article