Zebrafish Research
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Zebrafish testis has become a powerful model for reproductive biology of teleostean fishes and other vertebrates and encompasses multiple applications in applied and basic research. Many studies have…Read articleDLS Sample Preparation: Using U-Shaped Glass Capillaries for Sample Mounting
The TCS SP8 DLS microscope system from Leica Microsystems is an innovative concept which integrates the Light Sheet Microscopy technology into the SP8 confocal platform. Due to its unique optical…Read articleReal Time Observation of Neutrophil White Blood Cell Recruitment to Bacterial Infection In Vivo
The zebrafish (Danio rerio) is an emerging vertebrate model organism to study infection. The transparent larva comprises a fully functional innate immune system and enables live imaging of fluorescent…Read articleEvaluation of Zebrafish as a Model to Study the Pathogenesis of the Opportunistic Pathogen Cronobacter Turicensis
Application example of HyVolution Super-Resolution - Bacteria belonging to the genus Cronobacter spp. have been recognized as causative agents of life-threatening systemic infections, primarily in…Read articleWork More Efficiently in Developmental Biology With Stereo Microscopy: Zebrafish, Medaka, and Xenopus
Among the aquatic model organisms used in molecular and developmental biology the most prominent are the zebrafish (genus species: Danio rerio), medaka or japanese rice fish (genus species: Oryzias…Read articleInfection of Zebrafish Embryos with Intracellular Bacterial Pathogens
Transparent zebrafish embryos have proved useful model hosts to visualize and functionally study interactions between innate immune cells and intracellular bacterial pathogens, such as Salmonella…Read articleIntravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury
We describe a technique of microinjecting the aminoglycoside, gentamicin, into 2 days post-fetilization (dpf) zebrafish larvae to induce acute kidney injury (AKI). We also describe a method for whole…Read articlePatch Clamp Recordings from Embryonic Zebrafish Mauthner Cells
Mauthner cells (M-cells) are large reticulospinal neurons located in the hindbrain of teleost fish. They are key neurons involved in a characteristic behavior known as the C-start or escape response…Read articleOrgan Regeneration: An Unlikely Fish Tale
Spectacular discoveries in cardiac tissue regeneration are rapidly moving researchers closer to the goal of harnessing regenerative techniques to repair the human heart. Only eleven years ago, Dr.…Read articleFind the Needle in the Haystack
The obvious has been explored. These days, biologists strive to identify and analyze hidden and rare events. The task is tackled by automatically screening large numbers of objects – typically growing…Read articleDeep Tissue Imaging
Developmental biology using Multiphoton microscopy with