How to Radically Simply Workflows in Your Imaging Facility

Turnbull, Lynne; Thrasivoulou, Christopher

March 03, 2023

How to Radically Simply Workflows in Your Imaging Facility

MicaCam episode 9 - Video On Demand

In this episode of MicaCam, our special guest Dr Christopher Thrasivoulou from the University College London (UCL) will discuss the benefits of utilizing Mica from an Imaging Facility perspective. He will talk about automated and streamlined imaging workflows for complex biological systems. These help scientists to generate meaningful quantitative results with less time and effort. He will present an example of such a workflow by performing multi-color imaging of fluorescently labelled, fixed zebrafish embryos.

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Key Learnings

How to perform quantitative analysis of 4 different markers during time-lapse imaging and show the precise spatiotemporal order of events during apoptosis
How to increase the amount of data captured from one experiment using multiwell plates that enable multiple treatments to be samples at the same time
How to keep the environmental conditions stable to ensure healthy controls are maintained when exploring the effects of drug treatment on cells

Speakers

Dr. Lynne Turnbull

Principal Scientist - Leica Labs @ EMBL Imaging Center

Lynne is a Principal Scientist at Leica Microsystems. She received her PhD in Sydney Australia in cardiac biophysics and undertook postdoctoral training in San Francisco and Melbourne. Lynne’s research interests shifted to bacterial biofilms and motility, and she used different types of imaging to explore and understand how bacteria build communities and move through their environment. Upon moving to the University of Technology Sydney, Lynne established and managed the Microbial Imaging Facility. Lynne joined GE in 2016 to provide application support throughout Asia for super resolution microscopy. Since 2021 Lynne has been with Leica Microsystems based in the labs at the EMBL Imaging Center in Heidelberg.

Dr. Christopher Thrasivoulou

PhD. FRMS. Director of UCL’s Division of Biosciences Imaging Facility

Chris obtained his PhD from University College London in 1998 and is currently Director of UCL’s Division of Biosciences Imaging Facility. This large state-of-the -art imaging unit serves over 900 researchers from a very diverse range of science disciplines across the whole of UCL and number of external UK universities. The unit has 10 confocal microscopes, including super-resolution confocals, 2-Photon, SIM/PALM/STORM/TIRF, Lighsheet, High content imaging, Slide scanner, Raman and X-Ray CT. Chris’s research interest began in the field of neuroanatomy, studying the effects of aging and caloric restriction in the central and peripheral nervous system at the Royal Free Hospital School of Medicine. Chris moved to UCL in 2002, his research being focused on studying the role of connexins in wound healing and cancer cell biology and the role Wnt/calcium signalling in cancer aetiology.

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