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Multiplexed Imaging Types, Benefits and Applications

Understand the current options for multiplexed imaging techniques

Pancreatic ductal adenocarcinoma tissue section imaged with Cell DIVE  Pancreatic_ductal_adenocarcinoma_tissue_section_teaser.jpg

Multiplexed imaging is an emerging and exciting way to extract information from human tissue samples by visualizing many more biomarkers than traditional microscopy. By observing many biomarkers simultaneously, biological pathways previously explored only in isolation can be explored in concert, and complex tissue and cell phenotypes can be identified and probed. Many different methods for multiplexed imaging have become available, and each uses a different approach to achieve higher plexity.

About the article

In this article, we explore the different types of multiplexing technologies available, as well as their benefits and disadvantages. Specifically, we cover the main categories of multiplexed imaging: staining and imaging in one, one-pass lower-plex, all-in-one omics solutions, and iterative staining. We also compare these technologies based on their number of biomarkers, ease of use, multiple slides, biomarker selection flexibility and resolution. 

Additionally, we provide technical information, pros and cons, and background information so you can have a foundation to understand the current multiplexing landscape. Finally, we describe how Cell DIVE, our open multiplex solution, fits into the rapidly evolving multiplexing space, and resolves many of the pitfalls and challenges involved in executing a study.

Key Learnings

  • Explore the main categories of multiplexed imaging technologies available
  • Learn the positives and drawbacks of each approach
  • Learn how Cell DIVE overcomes many of the technical challenges of the field and delivers exceptional open multiplexing

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