However, to identify target sites in samples, high precision for localization of regions of interest is required. Currently, this is done mainly by cryo-confocal fluorescence microscopy, enabling high precision imaging in 3D. The confocal pinhole eliminates the out-of-focus light, increasing resolution and contrast, particularly in the axial direction.
In the following, it is explained how the LIGHTNING super-resolution technique and TauSense fluorescence lifetime-based tools are used under cryogenic conditions to enable better discrimination and identification of structures of interest with fluorescence microscopy which is performed for subsequent EM steps.
LIGHTNING super-resolution under cryo conditions
- How does it work?
- Deconvolution is well known, but what is special about LIGHTNING?
- How does LIGHTNING improve the targeting precision with the STELLARIS Cryo confocal microscope?
- Can I quantify my data after deconvolution with LIGHTNING?
TauSense under cryo conditions
- What is TauSense?
- How does TauSense work?
- Fully integrated into the Coral Cryo software workflow