Jan De Bock , Dr.
Jan studied biology and did his PhD in the field of olfaction, characterizing olfactory neurons in their response to odorants.
Jan has worked as a microscopy expert in different roles since 2003. He joined Leica Microsystems in 2011 as a product specialist for confocal microscopy. In 2017, he became member of the newly formed Workflow and Application Team responsible for correlative workflows, in particular involving sample preparation and imaging under cryogenic conditions.
New Imaging Tools for Cryo-Light Microscopy
New cryo-light microscopy techniques like LIGHTNING and TauSense fluorescence lifetime-based tools reveal structures for cryo-electron microscopy.
How to Target Fluorescent Structures in 3D for Cryo-FIB Milling
This article describes the major steps of the cryo-electron tomography workflow including super-resolution cryo-confocal microscopy. We describe how subcellular structures can be precisely located in…
How to Improve Live Cell Imaging with Coral Life
For live-cell CLEM applications, light microscopy imaging is a critical step for identifying the right cell in the right state at the right time. In this article, Leica experts share their insights on…
Targeting Active Recycling Nuclear Pore Complexes using Cryo Confocal Microscopy
In this article, how cryo light microscopy and, in particular cryo confocal microscopy, is used to improve the reliability of cryo EM workflows is described. The quality of the EM grids and samples is…
Crystal Clear Cryo Light-microscopy Images
This article describes how computational clearing of cryo light microscopy images improves the identification of cellular targets for cryo electron-microscopy.
Mosaic Images
Confocal laser scanning microscopes are widely used to create highly resolved 3D images of cells, subcellular structures and even single molecules. Still, an increasing number of scientists are…