Accurately Analyze Fluorescent Widefield Images

The specificity of fluorescence microscopy allows researchers to accurately observe and analyze biological processes and structures quickly and easily, even when using thick or large samples. However, out-of-focus fluorescence increases background, reduces contrast, and makes accurate image segmentation more challenging. A powerful solution to these issues is the use of THUNDER to remove image blur in combination with Aivia which automates widefield image analysis using AI to improve speed and accuracy. Here, we explore this synergetic approach in more detail.