STELLARIS STED & STELLARIS 8 STED STED-Mikroskope

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Five-color FLIM-STED with One Depletion Laser

Webinar on five-color STED with a single depletion laser and fluorescence lifetime phasor separation.
Five-color FLIM-STED with One Depletion Laser

A Versatile Palette of Fluorescent Probes

Researchers at the Max Planck Institute for Medical Research in Heidelberg have developed a general strategy to synthesize live-cell compatible fluorogenic probes, and the result are the new MaP (Max…
A Versatile Palette of Fluorescent Probes

STED sheds insight on mitochondrial protein synthesis

In this paper the authors have adapted a click chemistry-based method coupled with stimulated emission depletion nanoscopy (STED) to address these questions reporting that, in human cells in culture,…
STED sheds insight on mitochondrial protein synthesis

Mitochondrial Dynamics Quantitatively Revealed by STED Nanoscopy

Mitochondria are vital subcellular organelles, which can generate energy to support the entire lifecycle of biological cells. However, conventional fluorescence microscopy cannot provide a high enough…
Mitochondrial Dynamics Quantitatively Revealed by STED Nanoscopy

Benefits of Combining STED and Lifetime

In this interview, Professor Alberto Diaspro talks about the advantages of the White Light Laser and the TauSTED capabilities of STELLARIS 8 STED. He speaks about his experience with the confocal…
Benefits of Combining STED and Lifetime

Visualization of Submitochondrial Protein Distributions

By allowing visualization of submitochondrial protein distributions, fluorescence nanoscopy offers significant advantages for understanding cell death control. How cells manage, and control…
Visualization of Submitochondrial Protein Distributions

DNA Replication in Cancer Cells

DNA synthesis can be impeded by collisions between the DNA replication machinery and co-transcriptional R-loops leading to a major source of genomic instability in cancer cells. In this paper we…
DNA Replication in Cancer Cells

Kinetochore Assembly during Mitosis with TauSTED on 3D

Three-dimensional organization of the mitotic spindle together with the distribution of CENP-C and BUB1 based on TauSTED with multiple STED lines (592, 660 and 775 nm) can provide insights on…
Kinetochore Assembly during Mitosis with TauSTED on 3D

Regulators of Actin Cytoskeletal Regulation and Cell Migration in Human NK Cells

Dr. Mace will describe new advances in our understanding of the regulation of human NK cell actin cytoskeletal remodeling in cell migration and immune synapse formation derived from confocal and…
Regulators of Actin Cytoskeletal Regulation and Cell Migration in Human NK Cells

Upright Fluorescence Microscopy for Virus Replication Studies

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in late 2019 and spread over the world quickly. Due to its dramatic impact, researchers are interested in the nature of the…
Upright Fluorescence Microscopy for Virus Replication Studies

Mit LIGHTNING das Maximum an Informationen aus Ihrer Probe erhalten

LIGHTNING ist ein adaptiver Prozess zur Extraktion von Bildinformationen, bei dem vollautomatisch anderweitig nicht sichtbare Strukturen und feine Details sichtbar gemacht werden. Im Gegensatz zu…

Nanoscopy meets Lifetime: Introducing TauSTED

TauSTED combines the optical signals from STED and the photophysical information from the fluorescence lifetime at unprecedented speeds. This approach to STED uses phasor analysis in a novel way.…
Nanoscopy meets Lifetime: Introducing TauSTED

Universal PAINT – Dynamic Super-Resolution Microscopy

Super-resolution microscopy techniques have revolutionized biology for the last ten years. With their help cellular components can now be visualized at the size of a protein. Nevertheless, imaging…
Universal PAINT – Dynamic Super-Resolution Microscopy

Sample Preparation for GSDIM Localization Microscopy – Protocols and Tips

The widefield super-resolution technique GSDIM (Ground State Depletion followed by individual molecule return) is a localization microscopy technique that is capable of resolving details as small as…
Sample Preparation for GSDIM Localization Microscopy – Protocols and Tips

Super-Resolution GSDIM Microscopy

The nanoscopic technique GSDIM (ground state depletion microscopy followed by individual molecule return) provides a detailed image of the spatial arrangement of proteins and other biomolecules within…
Super-Resolution GSDIM Microscopy

Anwendungsbereiche

Super-Resolution Mikroskope

Als Pionier der Super-Resolution-Mikroskopie arbeitet Leica Microsystems mit Spitzenwissenschaftlern auf diesem Gebiet eng zusammen. Leica Microsystems ist das einzige Unternehmen, das sowohl…

Fortgeschrittene Mikroskopietechniken

Moderne Mikroskopietechniken umfassen sowohl hochauflösende als auch superauflösende Bildgebungsverfahren. Diese Techniken werden in erster Linie eingesetzt, um biologische Vorgänge mit extrem hoher…
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