Localization based super-resolution techniques such as STORM,
Universal PAINT – Dynamic Super-Resolution Microscopy
Localization Microscopy in Living Cells
Super-resolution microscopy techniques have revolutionized biology for the last ten years. With their help cellular components can now be visualized at the size of a protein. Nevertheless, imaging living cells is a challenge for most of the super-resolution principles. In this respect a technique called uPAINT (universal Point Accumulation for Imaging in Nanoscale Topography) caught attention. This single-molecule method utilizes the dynamic imaging of continuously labelled, arbitrary membrane biomolecules in living cells to reveal super-resolved images and single molecule trajectories at very high densities.