Christoph Greb , Dr.

Christoph Greb

Christoph Greb studied cell biology, parasitology and virology at the Philipps University in Marburg. In the course of his diploma thesis and his dissertation at the local Institute for Cytobiology and Cytopathology he examined the vesicular transport of apically destined proteins in polarized epithelial cells utilizing biochemistry as well as TIRF and confocal microscopy. From December 2011 he was writing for the Leica Science Lab as a freelancer. After his engagement for Novartis Vaccines & Diagnostics he started as Scientific Writer for the widefield team of Leica Microsystems in October 2013.

Studying Virus Replication with Fluorescence Microscopy

The results from research on SARS-CoV-2 virus replication kinetics, adaption capabilities, and cytopathology in Vero E6 cells, done with the help of fluorescence microscopy, are described in this…
Molecular structure of the green fluorescent protein (GFP)

Introduction to Fluorescent Proteins

Overview of fluorescent proteins (FPs) from, red (RFP) to green (GFP) and blue (BFP), with a table showing their relevant spectral characteristics.
Patch pipette touching a murine hippocampal neuron. Image courtesy of A. Aguado, Ruhr University Bochum, Germany.

What is the Patch-Clamp Technique?

This article gives an introduction to the patch-clamp technique and how it is used to study the physiology of ion channels for neuroscience and other life-science fields.
Neurons imaged with DIC contrast.

Differential Interference Contrast (DIC) Microscopy

This article demonstrates how differential interference contrast (DIC) can be actually better than brightfield illumination when using microscopy to image unstained biological specimens.

Going Beyond Deconvolution

Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…
Image of MDCK (Madin-Darby canine kidney) cells taken with phase contrast.

Phase Contrast and Microscopy

This article explains phase contrast, an optical microscopy technique, which reveals fine details of unstained, transparent specimens that are difficult to see with common brightfield illumination.

Immersion Objectives

How an immersion objective, which has a liquid medium between it and the specimen being observed, helps increase the numerical aperture and microscope resolution is explained in this article.
Intensity distribution (arbitrary color coding) of an image of two points where the distance between them corresponds to the Rayleigh criterion.

Microscope Resolution: Concepts, Factors and Calculation

This article explains in simple terms microscope resolution concepts, like the Airy disc, Abbe diffraction limit, Rayleigh criterion, and full width half max (FWHM). It also discusses the history.
Living HeLa cells stained with WGA-488 (yellow), SPY-Actin (cyan), and SiR-Tubulin (magenta). Instant Computational Clearing (ICC) was applied.

How to Perform Dynamic Multicolor Time-Lapse Imaging

Live-cell imaging sheds light on diverse cellular events. As many of these events have fast dynamics, the microscope imaging system must be fast enough to record every detail. One major advantage of…

Precise Spatial Proteomic Information in Tissues

Despite the availability of imaging-based and mass-spectrometry-based methods for spatial proteomics, a key challenge remains connecting images with single-cell-resolution protein abundance…
Image of murine-brain tissue showing a region removed with UV laser microdissection.

RNA Quality after Different Tissue Sample Preparation

The influence of sample preparation and ultraviolet (UV) laser microdissection (UV LMD) on the quality of RNA from murine-brain tissue cryo-sections is described in this article. To obtain good…
Cellular polarization dynamics before and after magneto-mechanical stimulation

Mechanically Stressed Cells under the Microscope

Traumatic brain injury, skin scarring, or fibrotic heart remodeling are examples for mechanical stress which cells and tissues are exposed to. In this article, the authors introduce a non-invasive,…
3D Reconstruction of brain slide image_Mica

3D Tissue Imaging: From Fast Overview To High Resolution With One Click

3D Tissue imaging is a widespread discipline in the life sciences. Researchers use it to reveal detailed information of tissue composition and integrity, to make conclusions from experimental…
MDCK cysts on day 9

How To Perform Fast & Stable Multicolor Live-Cell Imaging

With the help of live-cell imaging researchers gain insights into dynamic processes of living cells up to whole organisms. This includes intracellular as well as intercellular activities. Protein or…
Two-color caspase assay with tile scan

Multi-Color Caspase 3/7 Assays with Mica

Caspases are involved in apoptosis and can be utilized to determine if cells are undergoing this programmed cell death pathway in so-called caspase assays. These assays can be run by e.g. flow…

Fluorescent Dyes

A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescent agent. This can be a fluorescent protein – for example GFP –…
Spheroid shown here as a maximum projection of the raw widefield image data (left) and THUNDER image after Instant Computational Clearing (right). The images are derived from approximately 60 µm Z stacks. Different stains (alpha actin and vimentin) are used to help identify the various cell types. Images courtesy of Sandra Grijalva, Wallace H. Coulter Department of Biomedical Engineering, Georgia Tech and Emory University, Atlanta, USA.

Developing Heart Pacemaker Cells from Cardiac Spheroids

During the last decade, 3D cell culture has been established as a more realistic model compared to classical 2D culture systems. Cells can develop into miniature 3D objects, so called spheroids, which…
Dividing fission yeast S. pombe stained with two markers against spindle pole bodies (Pcp1-GFP, green) and cytokinesis ring (Rlc1-mCherry; red).

Studying Cell Division

Cell division is a biological process during which all cellular components must be distributed among the daughter cells. The division process requires firm coordination for success. Microscopy is…
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