Christoph Greb , Dr.
Christoph Greb studied cell biology, parasitology and virology at the Philipps University in Marburg. In the course of his diploma thesis and his dissertation at the local Institute for Cytobiology and Cytopathology he examined the vesicular transport of apically destined proteins in polarized epithelial cells utilizing biochemistry as well as TIRF and confocal microscopy. From December 2011 he was writing for the Leica Science Lab as a freelancer. After his engagement for Novartis Vaccines & Diagnostics he started as Scientific Writer for the widefield team of Leica Microsystems in October 2013.
Confluency Check with PAULA Cell Imager
Many cell-based experiments require cells in a certain state. This can include their morphology, fluorescent protein expression, and the confluency of the cell layer. Researchers check their cells…Read article
How to improve your DNA Mutation Analysis Workflow with Laser Microdissection
DNA mutations lead to abnormal proteins or missing functional proteins, which can cause cells to multiply uncontrollably and become cancerous. To find and understand the underlying mutation for a…Read article
Introduction to Mammalian Cell Culture
Mammalian cell culture is one of the basic pillars of life sciences. Without the ability to grow cells in the lab, the fast progress in disciplines like cell biology, immunology, or cancer research…Read article
What is Photomanipulation?
The term photomanipulation describes a wide range of techniques that enable the microscopist the transition from passive observer to instigator of events by offering a way of interacting with their…Read article
Photoactivatable, photoconvertible, and photoswitchable Fluorescent Proteins
Fluorescent proteins (FPs) such as GFP, YFP or DsRed are powerful tools to visualize cellular components in living cells. Nevertheless, there are circumstances when classical FPs reach their limits.…Read article
Milestones in Incident Light Fluorescence Microscopy
Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…Read article
Chronic Inflammation Under the Microscope
In the course of chronic inflammation certain body areas are recurrently inflamed. This goes along with many human diseases. With the help of widefield light microscopy, the underlying processes can…Read article
Gene Editing with CRISPR/Cas9 - Breakthrough in Genome Engineering
The CRISPR/Cas9 system is one of several different bacterial systems for defense against viral attacks. It consists of two main components. One is a small piece of RNA which binds to the viral target…Read article
Image Gallery: Application Options with Leica DM2500 LED Microscope
The number of applications in microscopy can be as manifold as life itself. Different specimens demand different contrast methods to get a decent result. Unstained cells normally do not show a very…Read article
Workflows & Protocols: Plant Laser Microdissection
During Leica workshops for LMD users in Brazil, hosted by the Federal University of Paraná/UFPR (UFPR) at the Centro de Energia Nuclear na Agricultura/USP (CENA), the power of laser microdissection…Read article
Workflows & Protocols: Laser Microdissection for Pathology and Cancer Research
Tumor development results from mutations in our DNA. For their deeper analysis, cancer researchers have to dissect the relevant tissue areas. Here we report the reason why laser microdissection is a…Read article
Definitions of Basic Technical Terms for Digital Microscope Cameras and Image Analysis
Most microscopes today are operated with a camera. The characteristics of the camera often decide whether the acquired image will reveal what a researcher wants to see. But when diving into camera…Read article
Infinity Optical Systems
“Infinity Optics” refers to the concept of a beam path with parallel rays between the objective and the tube lens of a microscope. Flat optical components can be brought into this “Infinity Space”…Read article
Introduction to Digital Camera Technology
A significant majority of modern optical microscopy techniques require the use of a digital camera. By working with digital devices researchers can observe specimens on a screen in real time or…Read article
Universal PAINT – Dynamic Super-Resolution Microscopy
Super-resolution microscopy techniques have revolutionized biology for the last ten years. With their help cellular components can now be visualized at the size of a protein. Nevertheless, imaging…Read article
GSDIM Publication List
GSDIM microscopy is a widefield super-resolution technique based on the localization of fluorophores with nanometer precision. With its help a lateral resolution of down to 20 nm can be achieved,…Read article
Immunotherapy to Combat Cancer: "Sleeping Beauty" – DNA Plasmid-based Gene Transfer System to Modify T Cells
Fighting cancer is a major goal of present-day medicine. So far mainly surgery, chemotherapy or radiation therapy are utilized to extinguish cancerous tissue, or at least set limits to it. …Read article
Video Interview with Jean-Luc Vonesch
Jean-Luc Vonesch is head of the imaging facility at the Institute of Genetics and Molecular and Cellular Biology (IGBMC), Strasburg, France. 23 years ago he was the founder of this facility which…Read article