Three-color TauSTED 660, 592 and 775 on mitotic cells immunostained for tubulin (cyan; 560-nm excitation), CENP-C (yellow; 488-nm excitation) and BUB1 (magenta; 647-nm excitation). Sample courtesy Carlos Sacristan Lopez, Hubrecht Institute,Utrecht*.
Images acquired on STELLARIS 8 STED with motCorr 93X glycerol STED WHITE objective lens and Power HyD X detectors.
*Details of how this sample is used to understand kinetochore assembly can be read in Sacristan, C. et al. Dynamic kinetochore size regulation promotes microtubule capture and chromosome biorientation in mitosis. Nat. Cell Biol. 20, 800–810(2018).