Contact & Support
Leica TCS SP8 X SP8 Configurable Confocal Leica Leica Microsystems

White Light Laser Confocal Microscope Leica TCS SP8 X

Optimization of multi-color image (1)

Primary culture of rat cortical neurons, excitation and detection settings were adjusted to optimal image contrast.

Structure Label Excitation [nm] Emission[nm]
Nucleus DAPI 405 424-465
Nestin (neuronal stem cells) cy2 495 504-545
DCX (immature neurons) cy3 560 559-624
Beta III-tubulin cy5 652 653-716

Optimization of multi-color image (2)

Paramecium stained with 7 dyes excitable in the visible range. Good separation and high image contrast for all 7 colors are possible due to the tunable excitation wavelengths.

Courtesy of Paolo Bianchini & Alberto Diaspro, Istituto Italiano di Tecnologia, Genoa, Italy

Removal of back scattered light by LightGate

LightGate completely removes back scattered light.
Sample: fixed HeLa cells, tubulin stained with BD Horizon V-500, nucleus stained with Chromeo 505. Images are recorded directly under excitation wavelength.

Full spectral characterization using LightGate

The rectangular lambda square plot displays full spectral information. This information is useful to investigate samples with complex or unknown fluorescence and to characterize new developed fluorophores.

The grey line within the graphical definition window indicates excitation = detection.