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Zebrafish High Content Screening Automation Leica HCS LSI

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Zebrafish

Zebrafish

Leica HCS LSI enables new perspectives of whole animal research. Large filed of view, extended working distance and adaptive zoom optics provide new insights into large specimen.

Danio rerio. Blood vessels in the fin at high resolution (right) and zoom in on detailed Zebrafish eye.

Courtesy of D. Hentsch et al., IGBMC, Illkirch, France.

Zebrafish, Brain (in vivo)

Transgenic embryo, Danio rerio; GFP; Epithalamus; Optic Tectum. The images illustrate the zoom flexibility of Leica HCS LSI: The optical zoom is used first, then a combination of optical and confocal zoom is applied. The image shows a maximum projection of a 3D stack using a Leica Z6 APO A optical zoom and 5x objective.

Courtesy of K. Palma, N. Guerrero, L. Armijo, ML. Concha, Laboratory of Experimental

Ontogeny (LEO), S. Härtel, Laboratory of Scientific Image Analysis (SCIANLAB). Anatomy and Developmental Biology Program, ICBM, Faculty of Medicine, University of Chile, Santiago, Chile.

Advanced Time Lapse: 4D Single Well Experiment Zebrafish Development

Advanced Time Lapse: 4D Single Well Experiment Zebrafish Development

Tracking the development of life over time offers exciting insights for embryogenesis – all in the same specimen. From egg to embryo, obtain exciting views of organ development with Leica HCS LSI software and see the backbone formation during the growth of a zebrafish.

Zebrafish, novocord development, Danio Rerio. Red: Rhodamine-dextran. Green: GFP, labeling of the notochord.

Courtesy of Sophie Dal-Pra, Team B&C Thisse, Imaging Centre of IGBMC, IGBMC, llkirch, France.

High content screening in 4D

High content screening in 4D

Multiwell assay

Brain research: automated 4D imaging discloses the spatio-temporal dynamics of fluorescent signals throughout development.

4D series of 2 day old zebrafish embryos, Danio rerio. Ventral views of embryonic brains of the ETvmat2:gfp stable transgenic line were automatically acquired every 38 min. GFP: neurons in the brain and expression in heart and blood vessels. The embryos were arrayed in 96-well plates. The regions of interest (brains) were manually selected using the Leica Mark & Find mode and imaged at high resolution.

Courtesy of J. Gehrig, KIT – Karlsruhe Institute of Technology, Institute of Toxicology and Genetics (ITG), Karlsruhe, Germany