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Leica EM UC7 Ultramicrotomes & Cryo-Ultramicrotomes Leica Leica Microsystems
  • Sample Preparation for Materials Science

    Sample preparation is key for observing the finest textures and details on the sample surface in high resolution. The right workflow and tools for the pre-preparation will lead to an excellently…
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  • Cell Culture

    Growing cells under lab conditions is the base for scientists working in fields of cell or developmental biology, cancer research, or any kind of life science and pharma research. Find out how Leica…
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  • Zebrafish Research

    For the best result during screening, sorting, manipulation, and imaging you need to see details and structures to make the right decisions for your next steps in research. Known for outstanding…
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  • Fluorescence Microscopy

    Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy, mainly because of its high sensitivity and high specificity. Find out how fluorescence…
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  • Geology, Environmental, Paleontology, and Earth Science Microscopes

    Leica microscopes for Geology, Environmental, Paleontology and Earth Science Research are powerful, modular imaging solutions for precise measurement and detailed analysis.
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  • Life Science Education

    As a teacher of biology, you show students how living organisms are structured using tissue sections and blood smears as microscope samples. To get the most out of their observations, they should be…
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  • Science Lab Topic: EM Sample Preparation

    Bridging Structure and Dynamics at the Nanoscale through Optogenetics and Electrical Stimulation

    Nanoscale ultrastructural information is typically obtained by means of static imaging of a fixed and processed specimen. However, this is only a snapshot of one moment within a dynamic system in…
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  • Science Lab Topic: EM Sample Preparation

    Superior Ultrastructural Preservation and Structural Contrast in Drosophila Tissue

    Optimal structural preservation of tissue can only be obtained by high pressure freezing. However, preparing the samples in optimal conditions is challenging. This article explains in detail how to…
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  • Science Lab Topic: EM Sample Preparation

    Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions

    Human peripheral blood contains RNA in cells and in extracellular membrane vesicles, microvesicles and exosomes, as well as in cell-free ribonucleoproteins. Circulating mRNAs and noncoding RNAs, being…
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  • Science Lab Topic: EM Sample Preparation

    Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS) of Yeast

    Application Note for Leica EM HPM100 - The sections are of yeast frozen with a Leica HPM100 high pressure freezer in the copper tube system, the cell paste was mixed with a pH 6.5 MES/dextran buffer…
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  • Science Lab Topic: EM Sample Preparation

    Immuno - Electron Microscopy of High Pressure Frozen and Freeze Substituted Mouse Heart

    Application Note for Leica EM AFS2 - Mouse heart tissue from wild-type (WT) and αT -catenin KO (KO) mice was excised, immersed in 20% (w/v) BSA and frozen immediately in a high-pressure freezer Leica…
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  • Science Lab Topic: EM Sample Preparation

    Cell Envelope Structure of a Gram-negative Thermotolerant y-Proteobacterium Acidithiobacillus sp., Strain HV2/2 and its Interaction with Pyrite

    Application Note for Leica EM AFS2 - Cells of the the moderately thermophilic Acidithiobacillus sp.strain HV2/2, were centrifuged at 20,0009xg and then cryo-immobilized by high-pressure freezing on…
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  • Science Lab Topic: EM Sample Preparation

    Symmetric Synapse - Clathrin Coated Endocytosis Pit in the Postsynaptic Dendrite

    Application Note for Leica EM ICE - WT hippocampal neurons were plated at a density of 80,000 cells/cm2 on 6 mm sapphire disks for 14 days. Sample were frozen using a high-pressure freezer (Leica EM
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  • Science Lab Topic: EM Sample Preparation

    Electron Microscopy of High Pressure Frozen and Freeze Substituted Arabidopsis Thaliana Root Tips Cells

    Application Note for Leica EM AFS2 - Arabidopsis thaliana roots (mutant PIN1pro:PIN1-GFP;bex5-1) were excised, immersed in 20% (w/v) BSA and frozen immediately in a high-pressure freezer. Freeze…
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  • Science Lab Topic: EM Sample Preparation

    Electron Microscopy Sample Preparation: “The Future is Cold, Dynamic and Hybrid”

    In 2014, the renowned Electron Microscopy for Materials Science (EMAT) research lab at the University Antwerp, Belgium, and Leica Microsystems started a fruitful collaboration to establish a Leica…
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  • Science Lab Topic: EM Sample Preparation

    Transmission Electron Microscopy Sample Preparation Protocols for the Ultrastructural Study of Cysts of Free-living Protozoa

    Cysts of free-living protozoa have an impact on the ecology and epidemiology of bacteria because they may act as a transmission vector or shelter the bacteria against hash environmental conditions.…
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  • Science Lab Topic: CLEM

    Correlative In-Resin Super-Resolution and Electron Microscopy Using Standard Fluorescent Proteins

    We introduce a method for correlative in-resin super-resolution fluorescence and electron microscopy (EM) of biological structures in mammalian culture cells. Cryo-fixed resin embedded samples offer…
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  • Science Lab Topic: EM Sample Preparation

    Influence of Tissue and Plant Species in the Trafficking of a Recombinant Protein in Plant Cells

    The development of recombinant DNA technology has allowed the use of plants for the production of biopharmaceuticals. In contrast to other production platforms, plants are unexpensive, easy to scale…
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  • Science Lab Topic: EM Sample Preparation

    Brief Introduction to Ultramicrotomy

    The usual thicknesses for transmission electron microscopic examinations range between 20 nm and 150 nm. Ultramicrotomy is a fast and clean method of producing ultra-thin sections of biological…
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  • Science Lab Topic: EM Sample Preparation

    Protein Trafficking in Cereal Seeds

    Seeds accumulate proteins and starch which will be broken down and mobilized upon germination. Among seeds, cereals constitute an example of a highly specialized storage tissue that constitutes up to…
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  • Science Lab Topic: EM Sample Preparation

    Perusing Alternatives for Automated Staining of TEM Thin Sections

    Contrast in transmission electron microscopy (TEM) is mainly produced by electron scattering at the specimen: Structures that strongly scatter electrons are referred to as electron dense and appear as…
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  • Science Lab Topic: EM Sample Preparation

    Atomic Force Microscopy Study of a Stretched Impact Copolymer

    Atomic force microscopy (AFM) is a powerful tool in the suite of nanoscale characterization techniques that provides a variety of information including topography, mechanical properties, and…
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  • Science Lab Topic: EM Sample Preparation

    Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue

    This protocol describes how biological samples, like brain tissue, can be imaged in three dimensions using the focussed ion beam/scanning electron microscope (FIB/SEM). The samples are fixed with…
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  • Science Lab Topic: CLEM

    Advanced Correlative Light/Electron Microscopy: Current Methods and New Developments Using Tokuyasu Cryosections

    Microscopy is an essential tool for analysis of cellular structures and function. With the advent of new fluorescent probes and super-resolution light microscopy techniques, the study of dynamic…
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