THUNDER Imaging Systems

THUNDER Imaging Systems

THUNDER Imaging Systems

To answer important scientific questions, they enable you to obtain a clear view of details, even deep within an intact sample, in real time without out-of-focus blur. Sharp imaging of 3D specimens is now as easy as working with your favorite camera-based fluorescence microscope. THUNDER Imagers with Computational Clearing define a new class of instruments for high-speed, high-quality imaging of thick, 3-dimensional specimens.

THUNDER Imaging Systems

The time has come for imaging systems that allow you to tackle easily biologically relevant 3D models: THUNDER Imagers.

To answer important scientific questions, they enable you to obtain a clear view of details, even deep within an intact sample, in real time without out-of-focus blur. Sharp imaging of 3D specimens is now as easy as working with your favorite camera-based fluorescence microscope. THUNDER Imagers with Computational Clearing define a new class of instruments for high-speed, high-quality imaging of thick, 3-dimensional specimens.

HeLa cell spheroid stained with Alexa Fluor 568 Phalloidin (Actin) and YOYO 1 iodide (Nucleus).

Decode 3D biology in real time*

Fundamentally change the way you work when imaging model organisms, tissue sections, and 3D cell cultures like organoids. Whether single cells, tissues, whole organisms, or tumor spheroids, THUNDER Imagers enable the decoding of 3D biology in real time.

THUNDER imaging systems excel due to

  • Delivery of benchmark performance and first-rate results for your application
  • Clear view of details even deep within the specimen thanks to Computational Clearing
  • Ease of use, speed, and sensitivity, just like with widefield imaging


*in accordance with ISO/IEC 2382:2015

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The THUNDER Technology

THUNDER is an opto-digital technology that uses the new Computational Clearing method to generate high resolution and high contrast images. Computational Clearing removes the typical haze inherent to all widefield images of thick samples. It produces brilliant results for large image stacks, as well as single images taken deep in your sample.

THUNDER, a Leica technology, automatically takes all relevant optical parameters into account in order to achieve haze-free results in real time.

Technology Note

Still questions about the THUNDER technology? The detailed answers are in this technology note.

Image of a locust ganglion, showing a maximum projection, without (left) and with (right) THUNDER. Sample thickness: 110µm, data volume: 376 MB. Acquisition time using Computational Clearing: 3 seconds.

Speed You Can See

The Computational Clearing method used by THUNDER works in real time. It starts right at the moment the image acquisition begins. Directly embedded in the image acquisition stream, THUNDER uses all the optical and operational parameters exploited during imaging.

This direct “optics handshake” ensures that thundered images appear stunningly fast on your screen and are ready for further evaluation! No need to wait until the experiment is finished.

THUNDER offers speed that you can see and feel.

Achieve Outstanding Results with Ease

THUNDER works fast. All it takes is one click. No need to calibrate the system or to adjust hardware components. Just select a Computational Clearing method and generate expert-level results in a breathtakingly short time.

Need to replicate an experiment for multiple specimens? All the imaging parameters of THUNDER can be easily reproduced and applied to other tasks.

THUNDER would specially be useful for time-lapse, because it allows very fast scanning of big samples in less than 2 minutes, and provides exceptionally crisp images.

Dr. Almary Guerra, Max Planck Institute for Heart and Lung Research, Bad Nauheim (Germany)

Computational Clearing

Computational Clearing efficiently differentiates between signal and background by taking the size of the targeted specimen features into account. This approach makes image details immediately visible which formerly were not revealed. Acquire one image and you have stunning results displayed instantly on the screen.

Depending on the type of application, the base method can be combined with deconvolution using the Leica decision mask technique. It is fully automated and works independently without manual user input. The technique delivers high quality images at very fast speed.

Reliable results, ready for the next step

Just activate Computational Clearing once and acquire haze-free images. It does not matter how simple or complex your experiments are, the raw data will always be stored for validation.

The haze free results with clear structures can easily be segmented and used for further analysis, such as counting nuclei, or the number of spots per nucleus, particle tracking, etc.

See through the haze

THUNDER Imagers remove the out-of-focus blur that occurs with widefield observation through the new Leica method called Computational Clearing. Now with THUNDER Imagers you can have both high-quality 3D images of thick samples and, at the same time, benefit from the speed and sensitivity like with a widefield system.

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