To answer important scientific questions, they enable you to obtain a clear view of details, even deep within an intact sample, in real time without out-of-focus blur. Sharp imaging of 3D specimens is now as easy as working with your favorite camera-based fluorescence microscope. THUNDER Imagers with Computational Clearing define a new class of instruments for high-speed, high-quality imaging of thick, 3-dimensional specimens.
Our experts for THUNDER imaging systems will be happy to advise you.
Fundamentally change the way you work when imaging model organisms, tissue sections, and 3D cell cultures like organoids.
*in accordance with ISO/IEC 2382:2015
The THUNDER Imager EM Cryo CLEM enables precise identification of cellular structures and smooth, secure transfer of coordinates, images, and samples through your correlative workflow.
The THUNDER Imager Model Organism allows fast and easy 3D exploration of whole organisms for developmental or molecular biology research.
THUNDER Imagers provide you with a solution for advanced 3D cell culture assays, whether you want to study stem cells, spheroids, or organoids.
THUNDER would specially be useful for time-lapse, because it allows very fast scanning of big samples in less than 2 minutes, and provide exceptionally crisp images.
Dr. Almary Guerra, Max Planck Institute for Heart and Lung Research, Bad Nauheim (Germany)
THUNDER is an opto-digital technology that uses the new Computational Clearing method to generate high resolution and high contrast images. Computational Clearing removes the typical haze inherent to all widefield images of thick samples. It produces brilliant results for large image stacks, as well as single images taken deep in your sample.
THUNDER, a Leica technology, automatically takes all relevant optical parameters into account in order to achieve haze-free results in real time.
Still questions about the THUNDER technology? The detailed answers are in this technology note.
THUNDER Imagers remove the out-of-focus blur that occurs with widefield observation through the new Leica method called Computational Clearing.
Now with THUNDER Imagers you can have both high-quality 3D images of thick samples and, at the same time, benefit from the speed and sensitivity like with a widefield system.
Whether single cells, tissues, whole organisms, or tumor spheroids, THUNDER Imagers enable the decoding of 3D biology in real time.
The Computational Clearing method used by THUNDER works in real time. It starts right at the moment the image acquisition begins. Directly embedded in the image acquisition stream, THUNDER uses all the optical and operational parameters exploited during imaging.
This direct “optics handshake” ensures that thundered images appear stunningly fast on your screen and are ready for further evaluation! No need to wait until the experiment is finished.
THUNDER works fast. All it takes is one click. No need to calibrate the system or to adjust hardware components. Just select a Computational Clearing method and generate expert-level results in a breathtakingly short time.
Need to replicate an experiment for multiple specimens? All the imaging parameters of THUNDER can be easily reproduced and applied to other tasks.
Computational Clearing efficiently differentiates between signal and background by taking the size of the targeted specimen features into account. This approach makes image details immediately visible which formerly were not revealed. Acquire one image and you have stunning results displayed instantly on the screen.
Depending on the type of application, the base method can be combined with deconvolution using the Leica decision mask technique. It is fully automated and works independently without manual user input. The technique delivers high quality images at very fast speed.
Just activate Computational Clearing once and acquire haze-free images. It does not matter how simple or complex your experiments are, the raw data will always be stored for validation.
The haze free results with clear structures can easily be segmented and used for further analysis, such as counting nuclei, or the number of spots per nucleus, particle tracking, etc.
We have re-imagined confocal imaging. See the World Premiere of our new Confocal Platform.
Application for wound healing assays
Leica Microsystems has now released version 8.2.1 of the LMS software for Laser Microdissection microscopes. Laser Microdissection is used to dissect...
Monitor your cell culture around the clock with PAULA and know the perfect time-point for your experiment
Find out how Leica supports your workflow and learn about other similar workflows. This publication provides a collection of most frequently used...
The new Center will support a combined mission to educate the next generation of scientists and drive new scientific discoveries
Ovarian cancer is one of the most severe types of cancers that women can suffer from during their lifetimes. It is the cancer’s tendency for frequent…
This list presents current publications in the major application fields for laser microdissection and is updated monthly.
To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using…
In this article you can learn how to optimize the preparation quality of your samples by using the ion beam etching method with the EM TIC 3X ion beam…
This webinar is designed to clarify crucial specifications that contribute to THUNDER Imagers' transformative visualization of 3D samples and…
Simultaneous visual and chemical analysis of steel non-metallic inclusions with a 2-methods-in-1 solution, using optical microscopy and laser induced…
In this article we explore several strategies you can take to improve the separation of fluorophores and increase the number of fluorescent probes you…
Here we present a webinar on the emerging applications of Stimulated Raman Scattering Microscopy, covering the following topics: Quantitative…
Leica Microsystems’ TauSense technology is a set of imaging modes based on fluorescence lifetime. Found at the core of the STELLARIS confocal…
This brochure presents and explains appropriate workflow solutions for the most frequently required sample preparation methods for material science…