Image collage of a dividing HeLa cell. Left cell shows the fluorescent signals H2B-mCherry and alphaTubulin-mEGFP to visualize microtubules and DNA. The right image shows the corresponding cell, imaged in a transmission electron microscope. Image collage of a dividing HeLa cell. Left cell shows the fluorescent signals H2B-mCherry and alphaTubulin-mEGFP to visualize microtubules and DNA. The right image shows the corresponding cell, imaged in a transmission electron microscope.

Leica Microsystems launches groundbreaking Live-Cell CLEM workflow

Wetzlar, Germany, 15.06.2021. — Leica Microsystems has today announced the launch of the Leica Nano workflow: a new live-cell correlative light and electron microscopy (CLEM) workflow solution designed to increase experimental success rates, improve reproducibility, and simplify light and electron microscopy (EM) integration.

The Leica Nano workflow combines high-contrast live cell imaging with EM sample preparation to provide a streamlined all-in-one workflow to answer a wide range of biological questions.

Understanding the basic building blocks of cells and tissue is a fundamental step in gaining better insights  into the underlying mechanism and potential treatments of fighting diseases like cancer, neurodegenerative disorders and viral infections such as COVID-19.  Here live-cell CLEM is providing a more complete picture in contrast to most workflows that are limited either by the sample range, the transfer time or the sample conditions during imaging and fixation – increasing complexity and risk of failure.

The Leica Nano workflow uses an intuitive, semi-automated three-step system to minimize the time between functional live-cell imaging and cryo fixation of the sample. A sample transfer between the light microscopy and the high-pressure freezer takes only a few seconds and results in a cryo fixed specimen. 
The time-specific sample transfer and fixation enables the investigation of dynamic events and link these to the nanometer resolution of the EM without compromising the health and integrity of the specimen.

On the light microscopy side Leica’s Thunder Imager provides state-of-the-art computational clearing technology to clean up the typical haziness of the 3D image. The Leica Nano workflow also introduces a new immersion objective corrected for sapphire glass provide optimal imaging conditions. The resulting images capture cellular functional and ultrastructural information that can be easily distinguished and integrated at the single-cell level.

Markus Lusser, President of Leica Microsystems, said: “Accelerating the pace of drug discovery and development to bring vital therapies to patients faster is one of our industry’s number one priorities as we look to the world beyond the COVID-19 pandemic. Everyone from the biggest biopharma companies to new biotech startups are striving to speed up science and are looking to innovative new technologies to provide the solution.

“The Leica Nano workflow will play a central role in helping to achieve this goal by reducing the complexities of workflows in the laboratory and helping scientists to achieve a reliably high experimental success rate for live cell CLEM – the only commercial solution on the market to be able to do this,” added Lusser. “We are extremely proud of this new solution – one of a number we have planned for launch this year – and excited to see the reaction its already getting from our customers and the marketplace.”

NOTES TO EDITORS

About Leica Microsystems
Leica Microsystems develops and manufactures microscopes and scientific instruments for the analysis of microstructures and nanostructures. Ever since the company started as a family business in the nineteenth century, its instruments have been widely recognized for their optical precision and innovative technology. It is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopes.

Find out more at Putting Dynamic Live Cell Data into the Ultrastructural Context.

Related Images

Image collage of a dividing HeLa cell. Left cell shows the fluorescent signals H2B-mCherry and alphaTubulin-mEGFP to visualize microtubules and DNA. The right image shows the corresponding cell, imaged in a transmission electron microscope.