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![An 8-color spectral unmixing result from a hyperspectral SRS (stimulated Raman scattering) dataset, showing the biochemically distinct structures of a fresh, untreated apple slice. An 8-color spectral unmixing result from a hyperspectral SRS (stimulated Raman scattering) dataset, showing the biochemically distinct structures of a fresh, untreated apple slice.](/fileadmin/_processed_/6/c/csm_8-color_spectral_unmixing_result_of_hyperspectral_SRS_dataset_of_apple_slice_d976f7bb1e.jpg)
How to Prepare Samples for Stimulated Raman Scattering (SRS) imaging
Find here guidelines for how to prepare samples for stimulated Raman scattering (SRS), acquire images, analyze data, and develop suitable workflows. SRS spectroscopic imaging is also known as SRS…
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![Multi-color SRS image of tri-cellular cancer spheroid showing total lipids (yellow, 2850 cm-1), unsaturated lipids (magenta, 3050 cm-1), collagen (SHG, cyan). Sample courtesy of R. Rudolf, J Klicks, Hochschule Mannheim Multi-color SRS image of a tri-cellular cancer spheroid](/fileadmin/_processed_/9/3/csm_Tricellular_Cancer_Model_bf9916586d.jpg)
The Potential of Coherent Raman Scattering Microscopy at a Glance
Coherent Raman scattering microscopy (CRS) is a powerful approach for label-free, chemically specific imaging. It is based on the characteristic intrinsic vibrational contrast of molecules in the…