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Label-free Vibrational Imaging of Cellular Structures in an Intact Zebrafish

Label-free Vibrational Imaging of Cellular Structures in an Intact Zebrafish

This overlay image shows the eye of an intact zebrafish.

  • Green: Stimulated Raman scattering (SRS) image of Lipid components (at 2850 cm-1).
  • Red: SRS image of Protein components (at 2935 cm-1).
  • Blue: second-harmonic signals, mainly from the sclera and cornea.

The different layers of the retina are clearly visible at cellular resolution. In addition, the protein-rich lens is shown (red), as well as second-harmonic signals from the sclera and cornea forming the encapsulation of the eye. Imaging within the eye of the intact animal is enabled by the nonlinear optical sectioning capabilities of SRS. Sample provided by Julien Vermot, Institute of Genetics and Molecular and Cellular Biology (IGBMC) Strasbourg, France, and Elena Remacha Motta, Leica MS and IGBMC. Objective: HC PL IRAPO 20x/0.75 W

 

Biology – in vivo imaging of food moth (Plodia interpunctella)

Biology – in vivo imaging of food moth (Plodia interpunctella)

This overlay image shows the surface of a larval silk gland.

  • Red: Lipid components (at 2850 cm-1).
  • Green: autofluorescence of chitin at 1064 nm (wavelength).

The middle top of the image shows a bristle of the larvae. Objective: HCX IR APO L25x/0.95 W 0.17.