Biology - In vivo imaging of food moth (Plodia interpunctella)
This overlay image shows the surface of a larval silk gland.
Red: Lipid components (at 2850 cm -1).
Green: autofluorescence of chitin at 1064 nm (wavelength).
The middle top of the image shows a bristle of the larvae.
Objective: HCX IR APO L25x/0.95 W 0.17.
Biology - In vivo imaging of food moth (Plodia interpunctella)
Surface of a larval silk gland, 10x magnification. Overlay image.
Red: fat cells which are located under the cuticula.
Green: Cuticula (on the right). The regular pattern of the cuticula is imaged by autofluorescence at a wavelength of 1064 nm. Long filaments surrounding the gland are bristles located at the surface of the larvae.
Image taken at 816 nm and 1064 nm with two channels. Objective: HC PL APO 10x/0.4 CS.
Biology - In vivo imaging of yeast cells
Images at two different wave numbers. Objective: HCX IR APO L25x/0.95 W 0.17.
Left: Yeast cells in one week old medium. The lipid droplets are small due to the lack of sugar. The bright blue parts are lipid droplets inside the yeast cells (at 2850 cm-1).
Right: Yeast cell cultured over three hours in fresh medium. The lipid droplets are bigger which indicates that there is enough sugar available for the cells. The dark blue parts are generated by water at a wave number of 3250 cm-1.
Biology - In vivo imaging of drosophila melanogaster larvae
Red: Fat cells imaged at a wave number of 2850 cm-1 (at 816 nm).
Green: Two different structures are displayed in green autofluorescence (at 1064 nm): Long tubes are parts of the tracheal system, striped matter in the background are muscles. Objective: HCX IR APO L25x/0.95 W 0.17.
Food - Fresh cheese and sandwich sauce
Left: CARS image of fresh cheese (left). Right: sandwich sauce (right).
The images look quite similar but a closer look reveals the differences: Fresh cheese is of more concrete consistence while; sandwich sauce is more fluid. This becomes evident in the distribution of the red lipid droplets.
Cosmetics
CARS images reveal details about properties of different cosmetical products. Left: hand cream. Right: body lotion.
The more fluid body lotion shows a rather even distribution of smaller lipid droplets while the hand cream shows bigger and more irregular lipid droplets.