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  • Live Cell Imaging

    Shifting perspective from single microscope components to a full working live cell imaging solution, Leica Microsystems integrates microscope, LAS X imaging software, cameras, and dedicated…
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  • Leica photomanipulation

    Photomanipulation

    The term photomanipulation encompasses a range of techniques that utilize the properties of fluorescent molecules to initiate events and observe how dynamic complexes behave over time in living cells.…
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  • Science Lab Topic: Fluorescence Microscopy

    What is Photomanipulation?

    The term photomanipulation describes a wide range of techniques that enable the microscopist the transition from passive observer to instigator of events by offering a way of interacting with their…
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  • Science Lab Topic: Fluorescence Microscopy

    Photoactivatable, photoconvertible, and photoswitchable Fluorescent Proteins

    Fluorescent proteins (FPs) such as GFP, YFP or DsRed are powerful tools to visualize cellular components in living cells. Nevertheless, there are circumstances when classical FPs reach their limits.…
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  • Science Lab Topic: Neuroscience

    Clap On, Clap Off. Protein On, Protein Off

    Unlike genetic or pharmacological manipulation, light-controlled proteins respond immediately, can be temporally and spatially triggered, are reversible, and are specific to the protein of interest.…
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  • Science Lab Topic: Neuroscience

    Video Talk by Karl Deisseroth: Optogenetics

    Optogenetics is a combination of genetics and optics to achieve a gain or loss of function of biochemical events such as action potentials in a particular neuron or tissue. Opsin genes encode proteins…
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  • Science Lab Topic: Neuroscience

    Optogenetic Toolkit Goes Multicolor

    Optogenetics is a technique that allows scientists to control neurons’ electrical activity with light by engineering them to express light-sensitive proteins. Within the past decade, it has become a…
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  • Science Lab Topic: Neuroscience

    Optogenetics

    Optogenetics is a technique that allows light-controlled responses of transfected cells. The cells are genetically modified by introduction of genes that code for light-induced channels or ion pumps.…
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  • Science Lab Topic: Confocal Microscopy

    Live-cell Imaging Techniques

    The understanding of complex and/or fast cellular dynamics is an important step for exploring biological processes. Therefore, today’s life science research is increasingly focusing on dynamic…
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  • Science Lab Topic: Confocal Microscopy

    Fluorescence Recovery after Photobleaching (FRAP) and its Offspring

    FRAP (Fluorescence recovery after photobleaching) can be used to study cellular protein dynamics: For visualization the protein of interest is fused to a fluorescent protein or a fluorescent dye. A…
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