Flexibility of imaging applications
To achieve the best imaging conditions, you can adjust the K5 camera flexible parameters to meet your sample needs:
- Image dynamic cellular events with up to 40 fps imaging
- Resolve detailed structures thanks to high 4.2 MP resolution
- Reduce exposure times and phototoxic effects with high 80% quantum efficiency.
Many commonplace CCD cameras have relatively low QE and high noise, which can reduce signal-to-noise and make it difficult to image structures of interest.
This image with DAPI-Nuclei (blue), Alexa488-Actin (green), MitoRed-mitochondria (red), and Alexa647-WGA (magenta) show the improvements that the Scientific CMOS technology in the K5 can give over CCD cameras.
Efficiently segment real time events
Get more biologically-relevant information with time lapse imaging thanks to the increased throughput powered by up to 40 fps observations.
Imaging cellular motility and protein dynamics in live cells requires high QE and fast frame rates. The K5‘s features make it an incredible camera for routine live cell imaging.
Here, U2OS cells expressing LifeAct-TagGFP2 were imaged for 150ms every 500ms for 5 minutes. Samples courtesy of ibidi GmbH, Gräfelfing, Germany.
Improve your images with better signal-to-background
Combine the K5‘s flexibility with the power of Computational Clearing.
Remove the haze from conventional widefield images accurately thanks to validated K5 and THUNDER Imager workflows.
The K5 and THUNDER Imager 3D Assay enables clear identification of Alpha (GFP-green) and Beta (mCardinal-red) cells within a developing zebrafish pancreas. Imaged in the blue (Hoechst), green (GFP), and red (mCardinal) channels, this 150-image z-stack was completed with all channels within a minute.
Images courtesy of Radhan Ramadass and Yu Hsuan at the Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.