Imaging Intracellular Temperature using FLIM

This video shows the presentation of Dr. Kohki Okabe given at the 9th Congress of the Federation of Asian and Oceanian Physiological Society (FAOPS) held in Kobe, Japan on 30 March 2019.

Dr. Okabe is an Assistant Professor and JST-PRESTO Researcher with the Laboratory of Bioanalytical Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo.

The power of lifetime contrast for the investigation of cellular physiology and dynamics can be fully harnessed with the SP8 FALCON integrated solution for fluorescence lifetime imaging microscopy (FLIM).

1. Hoshi Y, Okabe K, Shibasaki K, Funatsu T, Matsuki N, Ikegaya Y, Koyama R, Ischemic brain injury leads to brain edema via hyperthermia-induced TRPV4 activation., J. Neuroscience, 38 (25), 5700-5709 (2018).
2. Okabe K, Sakaguchi R, Shi B, Kiyonaka S, Intracellular thermometry with fluorescent sensors for thermal biology, Pflügers Archiv - European Journal of Physiology, 470, 717-730 (2018).
3. Okabe K, Inada N, Gota C, Harada Y, Funatsu T, Uchiyama S, Intracellular temperature mapping with a fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy. Nat. Commun., 3, 705 (2012).