Imaging Intracellular Temperature using FLIM

Video Presentation by Dr. Kohki Okabe

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In this presentation, Dr. Kohki Okabe explains how intracellular temperature distributions in live cells can be visualized with temperature sensors based upon fluorescence lifetime. His research group observed an intracellular temperature gradient between the nucleus and cytoplasm. By manipulating local temperatures inside living cells, they also discovered a unique cellular response, including acute translation reprogramming through RNA granule formation, revealing the existence of intracellular thermal signaling.

Video Presentation

This video shows the presentation of Dr. Kohki Okabe given at the 9th Congress of the Federation of Asian and Oceanian Physiological Society (FAOPS) held in Kobe, Japan on 30 March 2019.

Dr. Okabe is an Assistant Professor and JST-PRESTO Researcher with the Laboratory of Bioanalytical Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo.

The power of lifetime contrast for the investigation of cellular physiology and dynamics can be fully harnessed with the SP8 FALCON integrated solution for fluorescence lifetime imaging microscopy (FLIM).

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