Optimizing THUNDER Platform for High-Content Slide Scanning

With rising demand for full-tissue imaging and the need for FL signal quantitation in diverse biological specimens, the limits on HC imaging technology are tested, while user trainability and ease-of-use at core facilities becomes a matter of cost and efficiency. Here we demonstrate a feasible workflow developed for the THUNDER platform at our facilities, to support diverse research environments with needs ranging from KO-mouse tissue analysis to human cancer.

Topics & Tags

Speaker: Philipp P. Henrich , PhD

Senior Scientist and Lead of Microscopy Services, The Jackson Laboratory

Learning Objectives

  • Preview scans of multiple slides and fast tissue selection
  • Best workflow for using the AFC-assisted focus-map setup
  • Usage of camera sensitivity modes for fast acquisition
  • Post-processing of large datasets on the THUNDER workstation

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