Sciences de la vie

Sciences de la vie

Sciences de la vie

C'est ici que vous pourrez développer vos connaissances, vos capacités de recherche et les applications pratiques de la microscopie dans divers domaines scientifiques. Apprenez à obtenir une visualisation précise, à interpréter les images et à faire progresser la recherche. Trouvez des informations pertinentes sur la microscopie avancée, les techniques d'imagerie, la préparation des échantillons et l'analyse des images. Les sujets abordés comprennent la biologie cellulaire, les neurosciences et la recherche sur le cancer, en mettant l'accent sur les applications et les innovations de pointe.
Projection of a confocal z-stack. Sum159 cells, human breast cancer cells kindly provided by Ievgeniia Zagoriy, Mahamid Group, EMBL Heidelberg, Germany. Blue–Hoechst - indicates nuclei, Green–MitoTracker mitochondria, and red–Bodipy - lipid droplets

New Imaging Tools for Cryo-Light Microscopy

New cryo-light microscopy techniques like LIGHTNING and TauSense fluorescence lifetime-based tools reveal structures for cryo-electron microscopy.
Correlation of markers in the LM and the FIB image.

How to Target Fluorescent Structures in 3D for Cryo-FIB Milling

This article describes the major steps of the cryo-electron tomography workflow including super-resolution cryo-confocal microscopy. We describe how subcellular structures can be precisely located in…

Tomographie Cryoélectronique

La tomographie cryoélectronique (CryoET) est utilisée pour analyser les biomolécules dans leur environnement cellulaire avec une résolution sans précédent, inférieure au nanomètre.

Advancing Cellular Ultrastructure Research

Freeze-fracture and freeze-etching are useful tools for studying flexible membrane-associated structures such as tight junctions or the enteric glycocalyx. Freeze-fracture and etching are two…

The Cryo-CLEM Journey

This article describes the Cryo-CLEM technology and the benefits it can provide for scientists. Additionally, some scientific publications are highlighted. Recent developments in cryo electron…
Cryo FIB lamella - Overlay of SEM and confocal fluorescence image. Target structure in yeast cells (nuclear pore proteine Nup159-Atg8-split Venus, red) marked by an arrow. Scale bar: 5 µm. Alegretti et al.,  Nature 586, 796-800 (2020).

Targeting Active Recycling Nuclear Pore Complexes using Cryo Confocal Microscopy

In this article, how cryo light microscopy and, in particular cryo confocal microscopy, is used to improve the reliability of cryo EM workflows is described. The quality of the EM grids and samples is…

Studying the Microstructure of Natural Polymers in Fine Detail

The potential of cryogenic broad ion beam milling used in combination with scanning electron microscopy (cryo-BIB-SEM) for imaging and analyzing the microstructure of cryogenically stabilized soft…
Roland A. Fleck

Expert Knowledge on High Pressure Freezing and Freeze Fracturing in the Cryo SEM Workflow

Get an insight in the working methods of the laboratory and learn about the advantages of Cryo SEM investigation in EM Sample Preparation. Find out how high pressure freezing, freeze fracturing and…

Brief Introduction to Freeze Fracture and Etching

Freeze fracture describes the technique of breaking a frozen specimen to reveal internal structures. Freeze etching is the sublimation of surface ice under vacuum to reveal details of the fractured…
Scroll to top