Sciences de la vie
Sciences de la vie
C'est ici que vous pourrez développer vos connaissances, vos capacités de recherche et les applications pratiques de la microscopie dans divers domaines scientifiques. Apprenez à obtenir une visualisation précise, à interpréter les images et à faire progresser la recherche. Trouvez des informations pertinentes sur la microscopie avancée, les techniques d'imagerie, la préparation des échantillons et l'analyse des images. Les sujets abordés comprennent la biologie cellulaire, les neurosciences et la recherche sur le cancer, en mettant l'accent sur les applications et les innovations de pointe.
Ratiometric Imaging and Analysis of Ion Concentration in Cells
Many cellular functions depend on the dynamic balance of ions, electric potentials, and pH between the cytosol and surrounding extracellular space. Changes in these values affect cellular function.…
What is FRET with FLIM or as it is usually known FLIM-FRET?
Förster resonance energy transfer (FRET) is a well-established fluorescence-based technique which is used to study molecular interactions. It is useful for the analysis of protein-DNA and…
Brief Introduction to High-Pressure Freezing for Cryo-Fixation
Preparation of biological specimens for electron microscopy (EM) often requires cryo-fixation which does not introduce significant structural alterations of cellular constituents. A common method used…
The Guide to STED Sample Preparation
This guide is intended to help users optimize sample preparation for stimulated emission depletion (STED) nanoscopy, specifically when using the STED microscope from Leica Microsystems. It gives an…
How to Prepare Samples for Stimulated Raman Scattering (SRS) imaging
Find here guidelines for how to prepare samples for stimulated Raman scattering (SRS), acquire images, analyze data, and develop suitable workflows. SRS spectroscopic imaging is also known as SRS…
Understanding Clearly the Magnification of Microscopy
To help users better understand the magnification of microscopy and how to determine the useful range of magnification values for digital microscopes, this article provides helpful guidelines.
How Marine Microorganism Analysis can be Improved with High-pressure Freezing
In this application example we showcase the use of EM-Sample preparation with high pressure freezing, freeze substiturion and ultramicrotomy for marine biology focusing on ultrastructural analysis of…
What is the Patch-Clamp Technique?
This article gives an introduction to the patch-clamp technique and how it is used to study the physiology of ion channels for neuroscience and other life-science fields.
How to Determine Cell Confluency with a Digital Microscope
This article shows how to measure cell confluency in an easy and consistent way with Mateo TL, increasing confidence in downstream experiments.
