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Webinar

High-pressure freezing: Revealing functional mechanisms of synaptic transmission

Webinar On-Demand

Learn more about applying optogenetic stimulation in the EM ICE and how this technology has the potential to reveal structural and functional mechanisms of synaptic transmission. Get a detailed introduction of how optogenetic stimulation is applied to intact networks in acute brains slices and organotypic brain slice cultures from mice.

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Speakers

Frédéric Leroux

Advanced workflow specialist, Leica Microsystems

Dr. Carolina Borges-Merjane

Postdoctoral Fellow in the lab of Professor Peter Jonas at the Institute of Science and Technology (IST) Austria

Olena Kim

PhD Student in the lab of Professor Peter Jonas at the Institute of Science and Technology (IST) Austria

Learning Objectives

  • Preparing brain tissue (acute slices and organotypic slice cultures) with high-pressure freezing.
  • Strategies to optimize the freezing of brain tissue.
  • Strategies to optimize the light stimulation of brain tissue prepared with high-pressure freezing.
  • Optimized freeze substitution for brain tissue specimens after light stimulation.
  • Possible future applications for brain tissue specimens prepared with high-pressure freezing and observed with light-stimulation.