Speakers
Frédéric Leroux
Advanced workflow specialist, Leica Microsystems
Dr. Carolina Borges-Merjane
Postdoctoral Fellow in the lab of Professor Peter Jonas at the Institute of Science and Technology (IST) Austria
Olena Kim
PhD Student in the lab of Professor Peter Jonas at the Institute of Science and Technology (IST) Austria
Learning Objectives
- Preparing brain tissue (acute slices and organotypic slice cultures) with high-pressure freezing.
- Strategies to optimize the freezing of brain tissue.
- Strategies to optimize the light stimulation of brain tissue prepared with high-pressure freezing.
- Optimized freeze substitution for brain tissue specimens after light stimulation.
- Possible future applications for brain tissue specimens prepared with high-pressure freezing and observed with light-stimulation.
