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High-pressure freezing: Revealing functional mechanisms of synaptic transmission

Webinar on-demand


Learn more about applying optogenetic stimulation in the EM ICE and how this technology has the potential to reveal structural and functional mechanisms of synaptic transmission. Get a detailed introduction of how optogenetic stimulation is applied to intact networks in acute brains slices and organotypic brain slice cultures from mice.

Key Learnings

  • Preparing brain tissue (acute slices and organotypic slice cultures) with high-pressure freezing.
  • Strategies to optimize the freezing of brain tissue.
  • Strategies to optimize the light stimulation of brain tissue prepared with high-pressure freezing.
  • Optimized freeze substitution for brain tissue specimens after light stimulation.
  • Possible future applications for brain tissue specimens prepared with high-pressure freezing and observed with light-stimulation.

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