Improvement of Imaging Techniques to Understand Organelle Membrane Cell Dynamics

Communications, Corporate

February 15, 2021

Improvement of Imaging Techniques to Understand Organelle Membrane Cell Dynamics

Free webinar on-demand

Understanding cell functions in normal and tumorous tissue is a key factor in advancing research of potential treatment strategies and understanding why some treatments might fail. Single-cell analysis is crucial in biomedical research to unravel which cellular and molecular pathways are altered in complex diseases such as cancer.

In this webinar, Dr. Simona Paladino shows how improved fluorescence microscopy imaging techniques help researchers acquire more relevant data for the analysis of organelle membrane cell dynamics.

She presents techniques that helped to reveal that chemo-resistant ovarian cancer cells display higher levels of cholesterol (labelled with filipin dye) on the plasma membrane compared to their sensitive counterpart, and that intracellular filipin-dots colocalizing with lysosomes have significant increases in chemo-resistant cells, indicating that these cells take up much more cholesterol. This is consistent with higher expression of LDL receptor.

Key Learnings

How to easily detect lipids, e.g. cholesterol, in cells
What insights cellular imaging gives us into physio-pathological mechanisms
Novel tools to visualize cellular structures and processes

Speakers

Dr. Simona Paladino

Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II

Authors

Corporate Communications

Leica Microsystems

Watch the Webinar

First Name *

Last Name *

Email *

Phone *

Company/Institution *

Position *

Zip *

Country/Region *

Questions or Comments

I am interested in receiving information about products and services, virtual and in-person events such as workshops and webinars and educational content from Leica Microsystems GmbH, their representatives and other Danaher companies.
I understand that I may be contacted by phone or email and consent to such communication. *

Yes

No

By clicking on the SUBMIT button, I confirm that I have reviewed and agree with Leica Microsystems GmbH Terms of Use and their Privacy Policy. I also understand my privacy choices as they pertain to my personal data, as detailed in the aforementioned Privacy Policy under ‘’Your Privacy Choices’’.