Speaker
Dr. Simona Paladino, Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II
In this webinar, Dr. Simona Paladino shows how improved fluorescence microscopy imaging techniques help researchers acquire more relevant data for the analysis of organelle membrane cell dynamics.
She presents techniques that helped to reveal that chemo-resistant ovarian cancer cells display higher levels of cholesterol (labelled with filipin dye) on the plasma membrane compared to their sensitive counterpart, and that intracellular filipin-dots colocalizing with lysosomes have significant increases in chemo-resistant cells, indicating that these cells take up much more cholesterol. This is consistent with higher expression of LDL receptor.
Learning Objectives
- How to easily detect lipids, e.g. cholesterol, in cells
- What insights cellular imaging gives us into physio-pathological mechanisms
- Novel tools to visualize cellular structures and processes
