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Super-resolution live cell imaging in multicolor SP8 LIGHTNING

12 h confocal time-lapse video of dividing RPE cells

12 h confocal time-lapse video of dividing RPE cells stably expressing eGFP-tubulin (cyan) and mRFP-Histidine H2B (magenta). Courtesy of C. Gliech, Holland Labs, John Hopkins School of Medicine, Baltimore, US. The Holland lab is interested in the molecular mechanisms that control accurate chromosome distribution and the role that mitotic errors play in human health and disease.

Neonatal rat, cardiomyocytes.

Neonatal rat, cardiomyocytes.

Neonatal Rat, Cardiomyocytes. Blue: Nucleus, DAPI. Green: Titin m8, Cy2. Red: Titin 9D10, Cy3. B&W: Titin T12, Cy5. Courtesy of Dr. Elisabeth Ehler, King's College London, The Randall Centre for Cell and Molecular Biophysics, London, United Kingdom

Super-resolution live cell imaging in Multicolor

Host pathogen interaction in plant cells

Prestudy: Simultaneous monitoring of multiple organelle markers in leaf of transgenic Arabidopsis thaliana plants, 1024x1024, PL APO 40x 1.1 W. Leaf of Arabidopsis thaliana. Cyan: Nuclear localisation signal (N7), mTurquoise2. Green: Microtubules (MAP4), VENUS. Magenta: Peroxisomes (SKL), tagRFP-T. Red: Plasma membrane (LTI6b), mKate2. Sample Courtesy of Dr. Hassan Ghareeb, Prof. Dr. Volker Lipka, Department of Plant Cell Biology, University of Göttingen, Germany. Courtesy of Dr. Hasan Ghareeb, Prof. Dr. Volker Lipka, Department of Plant Cell Biology, University of Göttingen, Germany.

Mouse Colon

Mouse Colon. Role study of chemokine receptor CX3CR1 in intestinal inflammatory diseases. Green: Macrophages, GFP-CX3CR1. B&W: Vasculature, AF 647-CD31. Red: Blood platelets, PE-CD49B. 15 fps, 512 x 512 , xyzt, 375 x 375 µm, z: 1601.6 µm. Duration of recording 3 min 19 sec. Objective: 25x0.95 W Fluotar VISIR. Courtesy of W.Y. Lee, Calvin, Phoebe & Joan Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Canada.

Imaging with LAS X Navigator

Dorsal view of a mouse embryonic heart

Dorsal view of a mouse embryonic heart

Dorsal view of a mouse embryonic heart. Blue: Blood vessels, SMAcy3. Red: Membrane lymphatic vessel wall, Lyve1 633. Green: Nuclear lymphatic vessel, Prox1 cy5.5. Scale bar is 500 µm. Courtesy of Ghislaine Lioux, Miguel Torres Sanchez, Valeria R. Caiolfa, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) Madrid, Spain.

Paramecium tetraurelia

Paramecium tetraurelia

Paramecium tetraurelia. Basal Body AF 488 (black & white). Imaging using resonant scanner at maximum FOV and format (2495 x 2495). 8 x line averaging. Image width: 100 µm Sample Courtesy of Anne Aubusson-Fleury. Institute for Integrative Biology of the Cell (I2BC), French National Centre for Scientific Research (CNRS), Gif-sur-Yvette, France.

NAVIGATOR tile scan (3x2) of a whole mount of mouse diaphragm muscle

Blue: Nuclei, B&W: F-actin, Red: Nicotinic acetylcholine receptors, a principal postsynaptic component of neuromuscular junctions. Green: Tyrosine hydroxylase, a marker for sympathetic neurons. The video shows the spatial relationship between muscle fibers, sympathetic neurons, and neuromuscular junctions. Processes of sympathetic neurons partially run orthogonally to muscle fibers and in parallel to each other (lower part) or they line up with the long axis of muscle fibers and make contact with neuromuscular junctions (upper parts). LIGHTNING automatically reveals the finest details applying an adaptive process for extraction of hidden information in the image. Courtesy of Tatjana Straka and Rüdiger Rudolf, University of Applied Science, Mannheim.

Multicolor super-resolution

Multicolor super-resolution

Cos-7 cells. Cyan: Nucleus, DAPI. Green: Mitochondria, TOM20 Oregon green. Red: Tubulin, TMR. Gray: Actin, SiR. Courtesy of Urs Ziegler, Jana Döhner, Center for Microscopy and Image Analysis (UZH), University of Zürich. Switzerland.

Multicolor super-resolution

Multicolor super-resolution

Whole mount mouse diaphragm muscle was stained for nicotinic acetylcholine receptors, a principal postsynaptic component of neuromuscular junctions (red) and tyrosine hydroxylase, a marker for sympathetic neurones (green). The image shows a direct comparison between 3D-projections of a group of neuromuscular junctions with (right) or without (left) deconvolution using Lightning software. Upon deconvolution, a clearly enhanced definition of sympathetic neuronal processes and of the fine patterned structure of the postsynaptic distribution of acetylcholine receptors becomes evident. Note the close spatial vicinity between sympathetic neurones and neuromuscular junctions. Courtesy of Tatjana Straka and Rüdiger Rudolf, University of Applied Science, Mannheim.