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Super-resolution live cell imaging in multicolor SP8 LIGHTNING

Dorsal view of a mouse embryonic heart

Dorsal view of a mouse embryonic heart

Dorsal view of a mouse embryonic heart. Blue: Blood vessels, SMAcy3. Red: Membrane lymphatic vessel wall, Lyve1 633. Green: Nuclear lymphatic vessel, Prox1 cy5.5. Scale bar is 500 µm.

Courtesy of Ghislaine Lioux, Miguel Torres Sanchez, Valeria R. Caiolfa, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) Madrid, Spain.

NAVIGATOR tile scan (3x2) of a whole mount of mouse diaphragm muscle

Blue: Nuclei, B&W: F-actin, Red: Nicotinic acetylcholine receptors, a principal postsynaptic component of neuromuscular junctions. Green: Tyrosine hydroxylase, a marker for sympathetic neurons. The video shows the spatial relationship between muscle fibers, sympathetic neurons, and neuromuscular junctions. Processes of sympathetic neurons partially run orthogonally to muscle fibers and in parallel to each other (lower part) or they line up with the long axis of muscle fibers and make contact with neuromuscular junctions (upper parts).

LIGHTNING automatically reveals the finest details applying an adaptive process for extraction of hidden information in the image.

Sample courtesy of Tatjana Straka and Rüdiger Rudolf, University of Applied Science, Mannheim.

Host pathogen interaction in plant cells

Prestudy: Simultaneous monitoring of multiple organelle markers in leaf of transgenic Arabidopsis thaliana plants, 1024x1024, PL APO 40x 1.1 W. Leaf of Arabidopsis thaliana. Cyan: Nuclear localisation signal (N7), mTurquoise2. Green: Microtubules (MAP4), VENUS. Magenta: Peroxisomes (SKL), tagRFP-T. Red: Plasma membrane (LTI6b), mKate2.

Sample Courtesy of Dr. Hassan Ghareeb, Prof. Dr. Volker Lipka, Department of Plant Cell Biology, University of Göttingen, Germany. Courtesy of Dr. Hasan Ghareeb, Prof. Dr. Volker Lipka, Department of Plant Cell Biology, University of Göttingen, Germany.

Mouse Colon

Role study of chemokine receptor CX3CR1 in intestinal inflammatory diseases. Green: Macrophages, GFP-CX3CR1. B&W: Vasculature, AF 647-CD31. Red: Blood platelets, PE-CD49B. 15 fps, 512x512 , xyzt, 375 x 375 µm, z: 1601.6 µm. Duration of recording 3 min 19 sec. Objective: 25x0.95 W Fluotar VISIR.

Video courtesy of W.Y. Lee, Calvin, Phoebe & Joan Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Canada