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Coherent Raman Scattering Microscope STELLARIS 8 CRS

Images

Label-free Vibrational Imaging of Cellular Structures in an Intact Zebrafish

Label-free Vibrational Imaging of Cellular Structures in an Intact Zebrafish

This overlay image shows the eye of an intact zebrafish.

  • Green: Stimulated Raman scattering (SRS) image of Lipid components (at 2850 cm-1).
  • Red: SRS image of Protein components (at 2935 cm-1).
  • Blue: second-harmonic signals, mainly from the sclera and cornea.

The different layers of the retina are clearly visible at cellular resolution. In addition, the protein-rich lens is shown (red), as well as second-harmonic signals from the sclera and cornea forming the encapsulation of the eye. Imaging within the eye of the intact animal is enabled by the nonlinear optical sectioning capabilities of SRS. Sample provided by Julien Vermot, Institute of Genetics and Molecular and Cellular Biology (IGBMC) Strasbourg, France, and Elena Remacha Motta, Leica MS and IGBMC.

Objective: HC PL IRAPO 20x/0.75 W

Biology – in vivo imaging of food moth (Plodia interpunctella)

Biology – in vivo imaging of food moth (Plodia interpunctella)

This overlay image shows the surface of a larval silk gland.

  • Red: Lipid components (at 2850 cm-1).
  • Green: autofluorescence of chitin at 1064 nm (wavelength).

The middle top of the image shows a bristle of the larvae.

Objective: HCX IR APO L25x/0.95 W 0.17.

Videos

Label-free Imaging of Mouse Brain Tissue Exhibiting Alzheimer’s Pathology

This movie shows a z-stack of a 50-μm thick mouse brain slice.

  • Green: CARS image of Lipid components (at 2850 cm-1)
  • Blue: autofluorescence of intracellular vesicles likely containing lipofuscin

Myelinated neurites (lipid-rich tubular structures) and pathological lipid deposits (blob-shaped structures) are associated with Amyloid-β-plaques. Dark voids in the lipid image correspond to neuronal cell bodies. Samples provided by Dr. Martin Fuhrmann and Andrea Baral, German Center for Neurodegenerative Diseases, Bonn, Germany.

Objective: HC PL IRAPO 40x/1.1 W CORR

In vivo blood flow of mouse

Label-free imaging of movement of red blood cells in a living mouse at video rate.