Confocal Microscope Platform STELLARIS

Gentle live cell imaging

The goal of any experiment with a live specimen is to make detailed observations without influencing or damaging your sample. STELLARIS enables you to image for longer periods preserving physiological conditions and sample integrity thanks to the optimally tuned excitation and detection provided by the Power HyD detector family and WLL combination, since both excitation and detection are optimally tuned.

The high detection efficiency of our Power HyD detector family in the green/blue segment of the spectrum provides you with improved imaging capabilities for the most used fluorescent proteins, such as GFP or YFP, thanks to efficient signal acquisition at the lowest levels of illumination.

With STELLARIS you don’t need to compromise sample health to get great images. Whether you need long acquisition times for high-resolution 3D reconstructions or the highest frame rates to capture rapid dynamic events, our efficient signal detection ensures that you can perform the experiments that you need, while your samples are protected from photobleaching and phototoxicity.

Multiplex beyond spectral options

With the TauSeparation tool, you can differentiate species based not on their fluorescence spectra, but on their fluorescence lifetime. The reason for this is that fluorescence lifetime information is orthogonal to fluorescence intensity and may be used to distinguish dyes with similar, overlapping spectra.

Using fluorescence lifetime-derived information, TauSense opens huge possibilities for multiplexing, expanding the number of simultaneous detection channels that can be used in each experiment. Imagine a cell labeled with two different fluorescent probes with similar emission spectra. Using traditional confocal microscopy, it would be extremely challenging or impossible to differentiate them in the image. Using TauSeparation, these probes can be assigned to two different channels based on their different lifetimes and can therefore be visualized as two separate images coming from the photons collected in the same spectral detection window.

Learn more about TauSense

ImageCompass – Simple, even for complex experiments

Software should not hold you back when using your confocal microscope; it should make life easier. Our newly developed smart user interface ImageCompass makes the setting up complex experiments significantly easier.

ImageCompass features a drag-and-drop menu to add fluorophores and guides you through the setup multicolor experiments. It also has to ability to perform automated optimization of the excitation and detection parameters to give you maximum signal yield.

ImageCompass moves technical parameters to the background, leaving practical information in prominent positions and giving you a cockpit view of your experiment, so you always have the information you need to stay in control of your experiment.

With ImageCompass you also get intuitive access to functional information from TauSense and super-resolution with LIGHTNING. ImageCompass saves you time and adapts to your sample preparation, leaving you to focus on your experiment and giving you the productivity to do more.