Live Cell Imaging
Live cell microscopy techniques are fundamental to get a better understanding of cellular and molecular function. Today, widefield microscopy is the most common technique used to visualize cell dynamics and development over long times. Confocal microscopy is also a key tool to generate 3D images of structures and study highly dynamic cellular processes at high spatial and temporal resolution, keeping the specimen in a close to native state.
Neuroscience studies require a variety of confocal imaging techniques to allow the study of the nervous system. For example, Multiphoton and light sheet techniques allow the study of large and deep tissues, such as brain sections; STED super-resolution is key to address neuronal spines structure, synaptic plasticity, and protein interactions at the synapse level. Fluorescence lifetime imaging can report on microenvironment changes in calcium and pH.
Due to the diffraction limit of light, traditional confocal microscopy cannot resolve structures below ~240 nm. Super-resolution microscopy techniques, such as STED, PALM or STORM or some deconvolution processing methods, are used when enhanced resolution is needed to study structures and molecular events below the diffraction limit scale.
Visual analysis of animal and human tissues is critical to understand complex diseases such as cancer or neurodegeneration. From basic immunohistochemistry to intravital imaging, confocal microscopy and advanced modalities can provide an understanding of cells, biomolecules and their interactions within their environment.
Fluorescence multicolor microscopy, which is one aspect of multiplex imaging, allows for the observation and analysis of multiple elements within the same sample – each tagged with a different fluorescent dye – and the same experiment. This results in efficient experiments but also in more reliable and meaningful results to understand complex processes within cells and tissues. This gallery shows images from specimens tagged with multiple fluorescent probes obtained with THUNDER and the STELLARIS platform.
Fluorescence microscopy allows the study of changes occurring in tissue and cells during cancer development and progression. Techniques such as live cell imaging are critical to understand cancer progression and metastasis.
How to separate spectrally overlapping fluorophores using TauSeparation
TauSeparation is a powerful tool for multiplexing fluorescence signal. It allows separating spectrally overlapping fluorophores based on their fluorescence lifetime. During acquisition of fluorescence intensity, the distribution of the representative lifetime components is shown and allows the selection of the different species. TauSeparation enables multiplexing experiments beyond the spectral options.
From the big picture to super-resolution detail: LAS X Navigator meets TauSTED
With the LAS X Navigator interface, you can generate overviews of whole samples in a fast and easy way. You can directly interact with the first result and pick the areas of interest that might be the key to answer your biological question. This video guides you from setting up a Navigator experiment to the final super-resolved TauSTED result.
STELLARIS’ smart user interface ImageCompass
Fast and easy setup of confocal multicolor experiments. STELLARIS confocal microscopes re-imagine the acquisition workflow with the new, smart user interface ImageCompass. With very little training, the intuitive ImageCompass enables you to successfully run experiments from setup to result. All in one screen with all the needed controls at your fingertips. It also saves you time and adapts to your sample preparation letting you focus on your experiment. ImageCompass gives you the productivity to do more. It’s simple, even for complex experiments.
How to swiftly interact with large samples using the LAS X Navigator
The LAS X Navigator interface combines images of large samples showing various levels of detail into one single view. With the LAS X Navigator, you always have a clear orientation for your imaging experiment without having to handle multiple separate files. It allows you to navigate seamlessly from overview to high-resolution detail and find the relevant details in your sample. Get excellent research results quickly and benefit from the high productivity of the LAS X Navigator on STELLARIS.
STELLARIS. Confocal re-imagined.
STELLARIS gives you the power to see more. Obtain more reliable data to test your hypothesis with precision using the synergy of the Power HyD detectors, the optimized beam path and the next generation White Light Lasers (WLL). Gain the potential to explore a new dimension in your samples using TauSense and get instant access to lifetime-based information. STELLARIS gives you increased productivity to do more thanks to the user interface, ImageCompass. Acquire images with a few clicks, even for complex experiments.
Understanding cellular systems using life-time based information
Gain insight into cellular structure, dynamics, and function by capturing the live state of cells. Using the TauSense tools on STELLARIS you can sense environmental changes and gain access to functional information.