ATTO Rho6G DMPE Fluorescent Label 5 mg
Product No. AD-RHO6G-195
ATTO-Dye Labeled Phospholipids
In recent years the investigation of biological membranes, e.g. intracellular membranes of live cells, plasma membranes etc., has become a major subject of interest. As a result there is a growing demand for fluorescent dye-labeled lipids, in particular glycero-phospholipids, to be incorporated in biological membranes. ATTO-TEC offers a variety of glycero-phospholipids, carrying one or two fatty acid chains (lipophilic groups) and a phosphate ester residue (hydrophilic group).
ATTO-dye fluorescent phospholipids are labeled at the hydrophilic head group. After incorporation in, e.g., a plasma membrane (phospholipid bilayer) the fluorophore is located at the water/lipid interface of the membrane.
"ATTO-TEC is a wholly owned subsidiary of Leica Microsystems"
Currently we provide the phospholipids
- 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE),
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE),
- 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphoethanolamine (PPE)
- 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE), and
- 1,2-dilauroyl-sn-glycero-3-phosphoethanolamine (DLPE)
labeled with ATTO-dyes.
ATTO Rho6G
ATTO Rho6G is a new rhodamine dye, based on the well-known laser dye Rhodamine 6G. Characteristic features of the label are strong absorption, extraordinarily high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. After coupling to a substrate ATTO Rho6G carries a net electric charge of +1. The fluorescence is excited efficiently in the range 515 - 545 nm.
A suitable excitation source for ATTO Rho6G is the 532 nm line of the frequency-doubled Nd:YAG laser.
