EM GP2 Automatic Plunge Freezer
The updated version of the EM GP automatic plunge freezer is based on our motto ‘with the users for the user’ in combining performance and flexibility in a practically-relevant way.
The EM GP2 plunge freezes fluid or extremely thin samples spread on an electron microscopy grid into liquid ethane after removing excess fluid by automatic blotting.
Prior to freezing the sample is maintained in a temperature and humidity controlled environmental chamber, adjustable between +4°C and +60°C and room humidity to 99 %.
User Safety
Safe and reproducible preparation for single particle analysis and cryo-tomography.
- Special rapid condensing liquefier head for easy and save fill of secondary cryogen
- Fixed secondary cryogen position, only environmental chamber movement
Sample Safety
- Dry Nitrogen Gas Flow environment
- Sample Storage and Transfer in liquid face using transfer container with pre-cooled grid box
- Customized adapter for optimized and safe sample transfer from the automatic plunge freezer EM GP2 to different cryo TEMs
Fluid or Thin Sample Preparation
To prepare vitrified thin samples for cryo-TEM, including biological suspensions and industrial emulsions in both aqueous and inorganic solvents, the bare grid technique is used.
Reproducibility
Freezing grids reproducibly and safely using the EM GP2 automatic plunge freezer.
- Sensor blotting gives equal distributes surface on grid for perfect imaging results
- Temperature stability of secondary cryogen
- Temperature and humidity controlled protected environment
Reproducible Results Within Minutes
Micrographs A and B show images of microtubules acquired at 20000x (A) and 39000x (B) nominal magnification in the hiles of the perforated carbon film. The ice is free of exogenous contamination, the protofilaments and the individual tubulin subunits are clearly visible along the length of the microtubules.
Courtesy of Dr. Guenter Resch, Nexperion e.U. – Solutions for Electron Microscopy; Dr. Thomas Heuser and Marlene Brandstetter, Vienna, Biocenter Core Facilities GmbH
Connectivity
To be connected at any time at any place is today something which is taken for granted.
As a logical consequence Leica Microsystems started to connect systems for EM Sample Preparation to ensure your samples are protected during the complete workflow process.
Flexibility
Depending on your needs the EM GP2 automatic plunge freezer can be configured individually by using different blotting types and parameters:
- Sensor controlled blotting
- Automated or manual single, double and multiple blotting
- Environmental parameter – Temperature and Humidity
- Blotting Parameter – Pre-Blot, Blot, Post-Blot time, Blot and Transfer Positions
