In-depth understanding of cellular structural biology
Cryo Light Microscope THUNDER Imager EM Cryo CLEM
Fluorescence Microscopy
Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy for its high sensitivity and high specificity. Fluorescence is a form of luminescence that…
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Cell Culture
Growing cells under lab conditions is the base for scientists working in fields of cell or developmental biology, cancer research, or any kind of life science and pharma research. Find out how Leica…
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Cancer Research
Cancer is a complex and heterogeneous disease caused by cells deficient in growth regulation. Genetic and epigenetic changes in one or a group of cells disrupt normal function and result in…
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Cell Biology
When your research is centered on understanding the cellular basis of human health and disease, it is critical to investigate the cells of interest in spatiotemporal and molecular detail.…
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Virology
Do your research interests focus on viral infection and disease? Find out how you can gain insights into virology with solutions for imaging and sample preparation from Leica Microsystems.
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Model Organisms in Research
A model organism is a species used by researchers to study specific biological processes. They have similar genetic characteristics to humans and are commonly used in research areas such as genetics,…
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Science Lab Topic:
EM Sample Preparation
How to Successfully Perform Live-cell CLEM
The Leica Nano workflow provides a streamlined live-cell CLEM solution for getting insight bout structural changes of cellular components over time. Besides the technical handling described in the…
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Science Lab Topic:
EM Sample Preparation
How to Successfully Implement the Leica Nano Workflow
The live-cell CLEM workflow allows you to capture dynamic information related to a relevant biological process as it happens and put these observations into their ultrastructural context. The Leica…
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Science Lab Topic:
CLEM
Advancing Cellular Ultrastructure Research
Freeze-fracture and freeze-etching are useful tools for studying flexible membrane-associated structures such as tight junctions or the enteric glycocalyx. Freeze-fracture and etching are two…
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Science Lab Topic:
CLEM
The Cryo-CLEM Journey
This article describes the Cryo-CLEM technology and the benefits it can provide for scientists. Additionally, some scientific publications are highlighted.
Recent developments in cryo electron…
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Science Lab Topic:
EM Sample Preparation
Download EM Workflow Solutions Booklet
This publication is a compilation of appropriate workflows for the most frequently used sample preparation methods, like Correlative Methodologies, Optogenetics & Electro-Physiology, Surface Analysis,…
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Science Lab Topic:
EM Sample Preparation
Exploring the Structure and Life Cycle of Viruses
The SARS-CoV-2 outbreak started in late December 2019 and has since reached a global pandemic, leading to a worldwide battle against COVID-19. The ever-evolving electron microscopy methods offer a…
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Science Lab Topic:
CLEM
Targeting Active Recycling Nuclear Pore Complexes using Cryo Confocal Microscopy
In this article, how cryo light microscopy and, in particular cryo confocal microscopy, is used to improve the reliability of cryo EM workflows is described. The quality of the EM grids and samples is…
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Science Lab Topic:
CLEM
Advancing Cell Biology with Cryo-Correlative Microscopy
Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with…
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Science Lab Topic:
EM Sample Preparation
Workflows and Instrumentation for Cryo-electron Microscopy
Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron…
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Science Lab Topic:
THUNDER Imaging
An Introduction to Computational Clearing
Many software packages include background subtraction algorithms to enhance the contrast of features in the image by reducing background noise. The most common methods used to remove background noise…
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Science Lab Topic:
EM Sample Preparation
Coronavirus Replication in Host Cells
In this study, a molecular pore complex, which spans both membranes of SARS-CoV-2/COVID-19 virus-induced cytosolic vesicles and likely plays a role in replication, was visualized.
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Science Lab Topic:
CLEM
Crystal clear cryo light-microscopy images
This article describes how computational clearing of cryo light microscopy images improves the identification of cellular targets for cryo electron-microscopy.
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Science Lab Topic:
THUNDER Imaging
THUNDER Technology Note
So far, widefield microscopy was not suitable to image larger volumes, since the contrast of the recorded fluorescence image is reduced by the background (BG) mainly originating from out of focus…
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