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THUNDER Imager EM Cryo CLEM
In-depth understanding of cellular structural biology
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How to Successfully Perform Live-cell CLEM
The Leica Nano workflow provides a streamlined live-cell CLEM solution for getting insight bout structural changes of cellular components over time. Besides the technical handling described in the…
How to Successfully Implement Coral Life
The live-cell CLEM workflow allows you to capture dynamic information related to a relevant biological process as it happens and put these observations into their ultrastructural context. The Leica…
Advancing Cellular Ultrastructure Research
Freeze-fracture and freeze-etching are useful tools for studying flexible membrane-associated structures such as tight junctions or the enteric glycocalyx. Freeze-fracture and etching are two…
The Cryo-CLEM Journey
This article describes the Cryo-CLEM technology and the benefits it can provide for scientists. Additionally, some scientific publications are highlighted.
Recent developments in cryo electron…
Download EM Workflow Solutions Booklet
This publication is a compilation of appropriate workflows for the most frequently used sample preparation methods, like Correlative Methodologies, Optogenetics & Electro-Physiology, Surface Analysis,…
Exploring the Structure and Life Cycle of Viruses
The SARS-CoV-2 outbreak started in late December 2019 and has since reached a global pandemic, leading to a worldwide battle against COVID-19. The ever-evolving electron microscopy methods offer a…
Targeting Active Recycling Nuclear Pore Complexes using Cryo Confocal Microscopy
In this article, how cryo light microscopy and, in particular cryo confocal microscopy, is used to improve the reliability of cryo EM workflows is described. The quality of the EM grids and samples is…
Advancing Cell Biology with Cryo-Correlative Microscopy
Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with…
Workflows and Instrumentation for Cryo-electron Microscopy
Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron…
An Introduction to Computational Clearing
Many software packages include background subtraction algorithms to enhance the contrast of features in the image by reducing background noise. The most common methods used to remove background noise…
Coronavirus Replication in Host Cells
In this study, a molecular pore complex, which spans both membranes of SARS-CoV-2/COVID-19 virus-induced cytosolic vesicles and likely plays a role in replication, was visualized.
Crystal Clear Cryo Light-microscopy Images
This article describes how computational clearing of cryo light microscopy images improves the identification of cellular targets for cryo electron-microscopy.
Real Time Images of 3D Specimens with Sharp Contrast Free of Haze
THUNDER Imagers deliver in real time images of 3D specimens with sharp contrast, free of the haze or out-of-focus blur typical of widefield systems. They can even image clearly places deep inside a…
Fields of Application
Fluorescence
Find out how fluorescence microscopes from Leica Microsystems support your research. Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy for its…
Cancer Research
Cancer is a complex and heterogeneous disease caused by cells deficient in growth regulation. Genetic and epigenetic changes in one or a group of cells disrupt normal function and result in…
Cell Biology
When your research is centered on understanding the cellular basis of human health and disease, it is critical to investigate the cells of interest in spatiotemporal and molecular detail.…
Virology
Do your research interests focus on viral infection and disease? Find out how you can gain insights into virology with solutions for imaging and sample preparation from Leica Microsystems.
Model Organisms in Research
A model organism is a species used by researchers to study specific biological processes. They have similar genetic characteristics to humans and are commonly used in research areas such as genetics,…
Cryo-Electron Tomography
Cryo-electron tomography (CryoET) is used to resolve biomolecules within their cellular environment down to an unprecedented resolution below one nanometer.
Advanced Microscopy Techniques
Advanced microscopy techniques: Advanced microscopy techniques encompass both high-resolution and super-resolution imaging techniques. These techniques are primarily used to visualize biological…