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SP8 FALCON: a novel concept in fluorescence lifetime imaging enabling video-rate confocal FLIM

SP8 FALCON (FAst Lifetime CONtrast) is a fast and completely integrated fluorescence lifetime imaging microscopy (FLIM) confocal platform. SP8 FALCON delivers video-rate FLIM with pixel-by-pixel quantification, thanks to a novel concept for measuring fluorescence lifetimes built on fast electronics and sensitive spectral hybrid detectors. Photon arrival times are recorded at count rates typical for standard confocal imaging. The system has ultra-short dead time and powerful built-in algorithms for data acquisition and analysis. The deep integration of FLIM into the confocal platform provides easy access to complex FLIM experiments.

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Read the Application Note in Nature Methods:

Alvarez L, Widzgowski B, Ossato G, van den Broek B, Jalink K, Kuschel L, Roberti MJ & Hecht F:

Application Note: SP8 FALCON: a novel concept in fluorescence lifetime imaging enabling video-rate confocal FLIM

Video 1: Live 3D FLIM on a patient-derived organoid. Monitoring a cancer-derived increase of signal transduction activity in live organoids with an ERK FRET-FLIM biosensor. Functional information showing FRET efficiency of the ERK biosensor. Higher FRET efficiency indicates higher ERK activity.
Video 2: Fast Calcium oscillation with SP8 FALCON Fast Calcium oscillation in HeLa cells stimulated by histamine and loaded with Oregon Green 488 Bapta-1 AM are observed at video rate. Video: Courtesy of Kees Jalink, Bram van den Broek, Netherlands Cancer Institute.