Cell Biology Image Gallery

Cell biology images taken with Leica Microsystems instruments

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Cell biology studies the structure, function and behavior of cells, including cell metabolism, cell cycle, and cell signaling. Fluorescence microscopes are an integral part of a cell biologist toolkit. Widefield and confocal microscopes are used widely to visualize the details of complex structures in the cellular context.

Cell biology imaging with STELLARIS confocal platform

The STELLARIS family of confocal microscopes provides you with high-end solutions that enable the study of live cell specimens, detailed single molecule analysis or helps you maximize the results you get out of your experiments.

Live NE-115 cells expressing mNeonGreen-LifeAct

Live NE-115 cells expressing mNeonGreen-LifeAct are stained with MitoTracker Green, NucRed and SiR Tubulin.

2 intensity channels.

4 lifetime-based channels

Live NE-115 cells expressing mNeonGreen-LifeAct are stained with MitoTracker Green, NucRed and SiR Tubulin. Signals are acquired with 2 detectors for two fluorophores each. Intensity image shows in yellow and gray. Signal in each detector will be distingushed with TauSeparation tool. It results in 4 color image: Actin: LifeAct-mNeonGreen (left: yellow, right: red); mitochondria: MitoTracker Green (left: yellow, right: green); nuclei: NUC Red (left: gray, right: blue); and tubulin: SiR-tubulin (left: gray, right: magenta). Sample courtesy: Max Heydasch, University of Bern, and Spirochrome.

LIGHTNING image overlay shows the maximum projection of a z-stack from fixed U2OS cells labelled with 5 color: AF488 (Tubulin, grey), SPY555 (Actin, magenta), MitoTrackerRed (Lumen of Mitochondria, green), Atto 647N (TOM 20, Mitochondria, red), CF770 WGA (Membranes, cyan).​​ scale bar 10µm
Mitochondria-localized GFP in Magnaporthe Oryzae (Rice blast fungus)
TauContrast distinguishes same fluorescent protein in different cell compartments in fungi. Mitochondria-localized GFP in Magnaporthe Oryzae (Rice blast fungus). As a new cell forms (to the right), the older cells die and the GFP starts to be seen in the vacuole. We can see this in the GFP channel (left) but we also seen a nice TauContrast change with the AAT (average arriving time) being longer for GFP in the vacuole compared to the mitochondria. Sample provided by Shen Qing, Naweed Naqvi lab, Temasek Lifescience Laboratory, Singapore.

Kita mycelia expressing mCherry at the membrane and in the interior​

L form streptomyces
Kita mycelia with mCherry expressed at the membrane and in the interior of the bacteria. mCherry can be separated using the TauSeparation tool in Tausense, as it exhibits different lifetime components depending in where it is localized. Sample courtesy: Shraddha Shitut, Insitute of Biology, Leiden University.​

STED for malaria research

STED for malaria research
STED for malaria research: STED nanoscopy of merozoites invading erythrocytes. 3D STED image showing overlays of RON4 (magenta) with the proteins PfRh5 (left, green), PfRipr (middle, green), PfCyRPA (right, green). Nuclear staining with DAPI is shown in blue, and erythrocytic membrane is shown in gray. Scale bar: 1 µm. Image courtesy: Jennifer Volz, Alan Cowman, Walter and Eliza Hall Institute of Medical Research, Australia, and Marko Lampe, EMBL, Germany.

Three color STED imaging of Cos 7 cells

Three color STED imaging of Cos 7 cells
Three color STED imaging of Cos 7 cells stained with antibodies against NUP153 (green), TOM20 (magenta), and with SiR–actin (gray). Confocal (top) and STED (bottom). Scale bar: 2μm. Sample courtesy: Urs Ziegler and Jana Doehner, ZMB, University of Zurich, Switzerland.

TCell

Living T cell in suspension. 3D reconstruction of confocal and STED stacks.
Living T cell in suspension. 3D reconstruction of confocal and STED stacks. Image courtesy: Marco Fritsche, Mathias Clausen and Christian Eggeling, MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, UK.

Live-cell TauSTED showing three distinct cellular structures in U2OS cells

Tracking fast spatial distribution changes of multiple subcellular structures in live cells helps you better understand their localization changes under physiological and pathological conditions. Live-cell TauSTED showing three distinct cellular structures in U2OS cells. Labels were used for actin (SiR-actin, glow), microtubules (SPY555-tubulin, cyan), and membranes (WGA-CF4 88, green). TauSTED was performed with three dif ferent STED lines (775, 660, and 592 nm, respectively). Scale bar is 5 μm. SiR and SPY probes are available from Spirochrome and CF dyes from Biotium, Inc.

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