The STELLARIS family of confocal microscopes provides you with high-end solutions that enable the study of live cell specimens, detailed single molecule analysis or helps you maximize the results you get out of your experiments.
Live NE-115 cells expressing mNeonGreen-LifeAct are stained with MitoTracker Green, NucRed and SiR Tubulin. Signals are acquired with 2 detectors for two fluorophores each. Intensity image shows in yellow and gray. Signal in each detector will be distingu
Image acquired with STELLARIS STED.
TauSTED 775 resolves the intricate cytoskeleton network labeled with SiR-tubulin (glow - Spyrochrome), and trafficking vesicles labeled with CF594 (cyan - Biotium).
Image acquired with a STELLARIS 8 DIVE FALCON.
Image acquired with STELLARIS STED. Live cell TauSTED on U2OS cells, using labels for actin (SiR-actin, glow), microtubules (SPY555-tubulin, cyan), and membranes (CF488A coupled to WGA, green). SiR and SPY are available from Spirochrome. CF dyes are available from Biotium Inc. Scale bar: 10 μm
Cytoskeleton and membranes in live cell imaged with TauSTED
Image acquired with STELLARIS 8 FALCON with a single excitation line and a single detector. The 4 distinct signals were obtained with phasor separation. The use of a single laser line and single detector improves live imaging speed and lowers the laser dose. Grey: Intensity only. Colors: Merged phasor-separated signals. Scale bar: 20 μm. Tracking 4 neuronal types with 4 red fluorescent proteins on a single detector with FLIM.
3D confocal image of C. elegans neurons labelled with four red fluorescent proteins
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