Deep in vivo multicolor imaging


Label-free imaging: Second (SHG) and third harmonic generation (THG)

Mouse brain cortex. Thy1-eYFP cortical neurons, THG blood cells. Excitation: 1300 nm. Objective: IRAPO 25x1.0 W motCorr. 70 fps, 256x256 px. Sample courtesy of Kevin Keppler, Light Microscopy Center, DZNE, Bonn, Germany.