Label-free Differentiation of Microplastics

Phasor analysis of fluorescence lifetime data

Phasor analysis for fluorescence lifetime imaging microscopy (FLIM) is a powerful and fitting-free tool that provides a 2D graphical assessment of pixel-accurate lifetime distributions in a sample image. In biology and biomedical research, this tool is used in a variety of applications, such as protein-protein interactions, signaling events in cells, or to distinguish spectrally overlapping fluorophores. In this study, we show that phasor analysis is also suitable for the identification and differentiation of anthropogenic materials, such as autofluorescent microplastics. With this method a label-free, fast, and sensitive identification and differentiation of the fluorescence, lifetime populations can be established. This study also shows the possibility of visualizing variations in fluorescence lifetimes, depending on external parameters like incubation at high temperatures.


Topics & Tags

Read the full article:

A Monteleone, W Schary, F Wenzel, H Langhals and D Dietrich:

Label-free identification and differentiation of different microplastics using phasor analysis of fluorescence lifetime imaging microscopy (FLIM)-generated data


Interested to know more?

Talk to our experts. We are happy to answer all your questions and concerns.

Contact Us

Do you prefer personal consulting?

  • Leica Microsystems Inc.
    1700 Leider Lane
    Buffalo Grove, IL 60089 United States
    Office Phone : +1 800 248 0123
    Service Phone : 1 800 248 0223
    Fax : +1 847-236-3009

You will find a more detailed list of local contacts here.