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Following Multiple Events during Staurosporine Apoptosis

How adding additional markers to an apoptosis kit can significantly increase the amount of information - MicaCam Episode 07 - Video On Demand

Two-color caspase assay with tile scan. U2OS cells were treated with the nuclear marker DRAQ5 (magenta) and CellEvent™ (yellow). Two-color_caspase_assay_with_tile_scan.jpg

Commercially available kits to study the process of apoptosis, or programmed cell death, are used to measure the toxicity of chemicals or new potential drugs. In this episode of MicaCam, we show how adding additional markers to an apoptosis kit can markedly increase the amount of information a researcher can obtain from the same experiment. The simultaneous capture of all markers during time-lapse imaging allows the precise order of events during staurosporine-induced cell death to be quantitatively analysed. 

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Key Learnings

  • How to perform quantitative analysis of 4 different markers during time-lapse imaging and show the precise spatiotemporal order of events during apoptosis
  • How to increase the amount of data captured from one experiment using multiwell plates that enable multiple treatments to be samples at the same time
  • How to keep the environmental conditions stable to ensure healthy controls are maintained when exploring the effects of drug treatment on cells

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Speaker

Dr. Lynne Turnbull, Principal Scientist - Leica Labs @ EMBL Imaging Center

Lynne is a Principal Scientist at Leica Microsystems. She received her PhD in Sydney Australia in cardiac biophysics and undertook postdoctoral training in San Francisco and Melbourne. Lynne’s research interests shifted to bacterial biofilms and motility, and she used different types of imaging to explore and understand how bacteria build communities and move through their environment. Upon moving to the University of Technology Sydney, Lynne established and managed the Microbial Imaging Facility. Lynne joined GE in 2016 to provide application support throughout Asia for super resolution microscopy.  Since 2021 Lynne has been with Leica Microsystems based in the labs at the EMBL Imaging Center in Heidelberg.

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