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Spectroscopic Evaluation of Red Blood Cells

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Hemoglobinopathies are a major healthcare problem. This study presents a possible diagnostic tool for thalassemia which is based on confocal spectroscopy. This approach exploits spectral detection and white light laser excitation to acquire autofluorescence signals from red blood cells (RBCs).

The discrimination between healthy and diseased RBCs was performed with autofluorescence signals using 405 nm excitation and acquiring the emission from 425 nm to 790 nm. Three characteristics were calculated from the mean emission intensities at 502 nm, 579 nm, 628 nm, and 649 nm.

From the results shown in this study, spectral confocal microscopy could be a useful tool for the discrimination of healthy and thalassemia patients.

Figure: Intensity vs. wavelength (top) and images from autofluorescence of red blood cells (RBCs) (bottom) corresponding to 502 nm and 628 nm for the following samples: (a) TC1, from a control patient; (b) T1, HbH (severely diseased). It can be appreciated that cellular structures appear brighter in T1.

Read the full article:

Rey-Barroso L., Roldán M., Burgos-Fernández F.J., Gassiot S., Ruiz Llobet A., Isola I. & Vilaseca M..:

Spectroscopic Evaluation of Red Blood Cells of Thalassemia Patients with Confocal Microscopy: A Pilot Study

Sensors 2020, 20(14), 4039; https://doi.org/10.3390/s20144039

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