Live Cell Imaging Gallery

Live cell images taken with Leica Microsystems instruments

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Live cell microscopy techniques are fundamental to get a better understanding of cellular and molecular function. Today, widefield microscopy is the most common technique used to visualize cell dynamics and development over long times. Confocal microscopy is also a key tool to generate 3D images of structures and study highly dynamic cellular processes at high spatial and temporal resolution, keeping the specimen in a close to native state.

Live cell imaging with STELLARIS confocal platform

The STELLARIS platform provides you with solutions whether you need long acquisition times for high-resolution 3D reconstructions or the highest frame rates to capture rapid dynamic events.

Live NE-115 cells expressing mNeonGreen-LifeAct - STELLARIS

Live NE-115 cells expressing mNeonGreen-LifeAct are stained with MitoTracker Green, NucRed and SiR Tubulin.

2 intensity channels.

4 lifetime-based channels

Live NE-115 cells expressing mNeonGreen-LifeAct are stained with MitoTracker Green, NucRed and SiR Tubulin. Signals are acquired with 2 detectors for two fluorophores each. Intensity image shows in yellow and gray. Signal in each detector will be distingushed with TauSeparation tool. It results in 4 color image: Actin: LifeAct-mNeonGreen (left: yellow, right: red); mitochondria: MitoTracker Green (left: yellow, right: green); nuclei: NUC Red (left: gray, right: blue); and tubulin: SiR-tubulin (left: gray, right: magenta). Sample courtesy: Max Heydasch, University of Bern, and Spirochrome.

TCell

Live-cell TauSTED showing three distinct cellular structures in U2OS cells

Calcium waves in mammalian cells

Calcium oscillation after mechanical stimulation in mammalian cells loaded with OregonGreen 488. The response in individual cells is recorded as a change in TauContrast. Time series acquired at 4.5 fps, TauContrast traces in different cells (ROI selected in different color). Image size: 256 x 256 pixles, Rainbow LUT (TauContrast): 1.0-4.0ns

Fast 3D in vivo imaging of Nematostella vectensis

Fast 3D in vivo imaging of Nematostella vectensis (Cnidaria) showing endogenous signal for cilia, nematosomes and clusters of freely circulating cnidocytes (green) and dextran red fluorescence (magenta). Endogenous signals and fluorescence separated by TauGating on HyD S, 340 volumes acquired in 12 min 45 s. Scale bar, 50 μm. Sample courtesy of Anniek Stokkerman and Aissam Ikmi, EMBL Heidelberg.

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