Live Cell Imaging Gallery

June 25, 2021

Live Cell Imaging Gallery

Live cell images taken with Leica Microsystems instruments

Nematostella Nematostella-LiveImaging-Stellaris.jpg

Live cell microscopy techniques are fundamental to get a better understanding of cellular and molecular function. Today, widefield microscopy is the most common technique used to visualize cell dynamics and development over long times. Confocal microscopy is also a key tool to generate 3D images of structures and study highly dynamic cellular processes at high spatial and temporal resolution, keeping the specimen in a close to native state.

Live cell imaging with STELLARIS confocal platform

The STELLARIS platform provides you with solutions whether you need long acquisition times for high-resolution 3D reconstructions or the highest frame rates to capture rapid dynamic events.

Zebrafish heart muscle cells and blood flow in transmission

Heart muscle cells (GFP) allow to follow the heartbeat dynamics. The transmission allows to observe the corresponding blood flow. Video acquired with DLS light sheet imaging, with fluorescence and transmission.

Transcription activity in mice gastric stem cell derived organoid

Hoechst channel labelling the nucleus. The differences in fluorescence lifetime corresponds to difference in DNA compaction. The longer lifetimes (red) correspond to cells with more open reading frames and an active DNA transcription such as stem cells. Video acquired with multiphoton (DIVE) and lifetime (FALCON).

Gentle 3D live imaging of Hydractinia symbiolongicarpus

Hydractinia expressing eGFP under the control of neurogenesis-related genes label. Imaged with DLS and LIGTHNING.

Endocytic pathway and mitochondria dynamics

Mitochondria labelled with MitoView Green. Membrane vesicles labelled with CellBright NIR750. The distinct endocytic steps are revealed with TauSeparation with lifetime component separation based on pH maturation steps correlated to endocytic maturation of vesicles

3D live Imaging of Spirogyra sp.

3D live Imaging of Spirogyra sp. Image acquired with DLS and LIGHTNING.
Endogeneous fluorescence of chloroplasts in green algea Spirogyra sp.Xyz-stack 3D-animation with STELLARIS 3D-viewer depth color coding.

Zebrafish heart muscle cells and blood flow in transmission

Heart muscle cells (GFP) allow to follow the heartbeat dynamics. The transmission allows to observe the corresponding blood flow. Video acquired with DLS light sheet imaging, with fluorescence and transmission.

Volumetric image of a Zebrafish beating heart

Heart muscle cells (GFP) allow to follow the heartbeat dynamics. The transmission allows to observe the corresponding blood flow. Video acquired with DLS light sheet imaging, with fluorescence and transmission.

Exploring Chloroplast activity with multiphoton and fluorescence lifetime imaging

Image acquired with a STELLARIS 8 DIVE FALCON.

The multiphoton transmission image shows the leaf structure, the endogenous chloroplast fluorescence is shown in green. Then the Fluorescence lifetime imaging fingerprint from a phasor analysis converts this endogenous to colors depending on the oxidative potential for each chloroplast.

Cytoskeleton and membranes in live cell imaged with TauSTED

Image acquired with STELLARIS STED.

Cytoskeleton and membranes in live cell imaged with TauSTED.

3D confocal image of C. elegans neurons labelled with four red fluorescent proteins.

Image acquired with STELLARIS 8 FALCON with a single excitation line and a single detector. The 4 distinct signals were obtained with phasor separation. The use of a single laser line and single detector improves live imaging speed and lowers the laser dose. Grey: Intensity only. Colors: Merged phasor-separated signals. Scale bar: 20 μm. Tracking 4 neuronal types with 4 red fluorescent proteins on a single detector with FLIM.

3D confocal image of C. elegans neurons labelled with four red fluorescent proteins

Cytoskeleton and membranes in live cell imaged with TauSTED

Image acquired with STELLARIS STED. Live cell TauSTED on U2OS cells, using labels for actin (SiR-actin, glow), microtubules (SPY555-tubulin, cyan), and membranes (CF488A coupled to WGA, green). SiR and SPY are available from Spirochrome. CF dyes are available from Biotium Inc. Scale bar: 10 μm

Live cell TauSTED on U2OS cells - confocal

Cytoskeleton and membranes in live cell imaged with TauSTED

Fast 3D in vivo imaging of Nematostella vectensis

Fast 3D in vivo imaging of Nematostella vectensis (Cnidaria) showing endogenous signal for cilia, nematosomes and clusters of freely circulating cnidocytes (green) and dextran red fluorescence (magenta). Endogenous signals and fluorescence separated by TauGating on HyD S, 340 volumes acquired in 12 min 45 s. Scale bar, 50 μm. Sample courtesy of Anniek Stokkerman and Aissam Ikmi, EMBL Heidelberg.

Calcium waves in mammalian cells

Calcium oscillation after mechanical stimulation in mammalian cells loaded with OregonGreen 488. The response in individual cells is recorded as a change in TauContrast. Time series acquired at 4.5 fps, TauContrast traces in different cells (ROI selected in different color). Image size: 256 x 256 pixles, Rainbow LUT (TauContrast): 1.0-4.0ns

Live NE-115 cells expressing mNeonGreen-LifeAct - STELLARIS

Live NE-115 cells expressing mNeonGreen-LifeAct are stained with MitoTracker Green, NucRed and SiR Tubulin. Signals are acquired with 2 detectors for two fluorophores each. Intensity image shows in yellow and gray. Signal in each detector will be distingu