Super-Resolution Microscopy Image Gallery

Super-resolution microscopy images taken with Leica Microsystems instruments


Due to the diffraction limit of light, traditional confocal microscopy cannot resolve structures below ~240 nm. Super-resolution microscopy techniques, such as STED, PALM or STORM or some deconvolution processing methods, are used when enhanced resolution is needed to study structures and molecular events below the diffraction limit scale.

Super-Resolution imaging with STELLARIS confocal platform

The STELLARIS confocal platform gives access to super-resolution with LIGHTNING and STED technologies for visualizing structures with greater precision and uncovering details that would otherwise not be visible.

Single molecule in-situ hybridization (smFISH) of RNA in Drosophila embryo whole-mount preparation

Living T cell in suspension

Cytoskeleton and membranes in live cell imaged with TauSTED

Image acquired with STELLARIS STED.

Cytoskeleton and membranes in live cell imaged with TauSTED - Confocal Cytoskeleton and membranes in live cell imaged with TauSTED - STED

TauSTED 775 resolves the intricate cytoskeleton network labeled with SiR-tubulin (glow - Spyrochrome), and trafficking vesicles labeled with CF594 (cyan - Biotium).

Cytoskeleton and membranes in live cell imaged with TauSTED

Image acquired with STELLARIS STED. SiR and SPY are available from Spirochrome. CF dyes are available from Biotium Inc.

Live cell TauSTED on U2OS cells - confocal Live cell TauSTED on U2OS cells - sted

Live cell TauSTED on U2OS cells, using labels for actin (SiR-actin, glow), microtubules (SPY555-tubulin, cyan), and membranes (CF488A coupled to WGA, green). Scale bar: 10

STED for malaria research

Three color STED imaging of Cos 7 cells

3D STED 775 deep nanoscopy of glomerulus in cleared kidney tissue

STED and DNA origami imaging

TauSTED 660 at extremely low STED power on COS7 cells immunostained for nuclear pores (NUPs) with AF555

STED for Microbiology

Image acquired with STELLARIS confocal platform.

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