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Super-Resolution Microscopy Image Gallery

Super-resolution microscopy images taken with Leica Microsystems instruments

LiveCell_TwoColor_STED.jpg

Due to the diffraction limit of light, traditional confocal microscopy cannot resolve structures below ~240 nm. Super-resolution microscopy techniques, such as STED, PALM or STORM or some deconvolution processing methods, are used when enhanced resolution is needed to study structures and molecular events below the diffraction limit scale.

Super-Resolution imaging with STELLARIS confocal platform

The STELLARIS confocal platform gives access to super-resolution with LIGHTNING and STED technologies for visualizing structures with greater precision and uncovering details that would otherwise not be visible.

TauSTED Xtend delivers details at the nanoscale level on-the-fly

Supplementary movie 1 from application note “Gentle, Multicolor Live imaging at the Nanoscale with TauSTED Xtend". Download the application note now.

TauSTED Xtend in action. The movie shows how TauSTED Xtend works with a single click, on-the-fly, as image acquisition takes place. COS7 cells stained with antibodies against NUP proteins located in the central basket (Map 414) and labeled with AF555: confocal (left panel) and TauSTED Xtend 660 (right panel).

Gentle multicolor live cell TauSTED Xtend 775

Supplementary movie 2 from application note “Gentle, Multicolor Live imaging at the Nanoscale with TauSTED Xtend". Download the application note now.

Two color, live cell Tau STED Xtend 775 of COS7 cells following cytoskeleton (left) and vesicles (right) dynamics. Cells labeled with SiR-tubulin (Spirochrome) and WGA-CF594 (Biotium). Low light dose for both excitation and STED allows for gentle long-term imaging (600 frames, 2s/frame).

Long term TauSTED Xtend on green fluorescent proteins

Supplementary movie 3 from application note “Gentle, Multicolor Live imaging at the Nanoscale with TauSTED Xtend". Download the application note now.

Live cells expressing LifeAct-mNeonGreen. TauSTED Xtend 589. 6 k format, 30 min STED imaging. Sample courtesy: M. Heydasch, Institute of Cell Biology, University of Bern, Switzerland.

Two-color TauSTED Xtend 589 of the inner ear section of a mouse

Two-color TauSTED Xtend 589 of the inner ear section of a mouse
Two-color TauSTED Xtend 589 of the inner ear section of a mouse. AF 488 and TauSTED Xtend 775 on AF 633-Phalloidin. Sample courtesy: Dennis Derstrof, Klinik fuer Hals-, Nasen- und Ohrenheilkunde, Universität Marburg, Germany & Prof. Dr. Dominik Oliver aus dem Institut für Physiologie und Pathophysiologie, Abteilung für Neurophysiologie, Universität Marburg, Germany.

Single molecule in-situ hybridization (smFISH) of RNA in Drosophila embryo whole-mount preparation

Single molecule in-situ hybridization (smFISH) of RNA in Drosophila embryo whole-mount preparation
Single molecule in-situ hybridization (smFISH) of RNA in Drosophila embryo whole-mount preparation. Probes are directly labeled (no signal amplification). Top: Three color TauSTED 775 of hb CalFluor 610 (cyan), gt Quasar 670 (green), eve Quasar 705 (magenta). Bottom: confocal overview of the Drosophila embryo preparation. Sample courtesy: Tom Pettini, University of Manchester, UK.

Living T cell in suspension

Living T cell in suspension. 3D reconstruction of confocal and STED stacks.
3D reconstruction of confocal and STED stacks. Image courtesy: Marco Fritsche, Mathias Clausen and Christian Eggeling, MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, UK.

Cytoskeleton and membranes in live cell imaged with TauSTED

Image acquired with STELLARIS STED.

Cytoskeleton and membranes in live cell imaged with TauSTED - Confocal Cytoskeleton and membranes in live cell imaged with TauSTED - STED

TauSTED 775 resolves the intricate cytoskeleton network labeled with SiR-tubulin (glow - Spyrochrome), and trafficking vesicles labeled with CF594 (cyan - Biotium).

Cytoskeleton and membranes in live cell imaged with TauSTED

Image acquired with STELLARIS STED. SiR and SPY are available from Spirochrome. CF dyes are available from Biotium Inc.

Live cell TauSTED on U2OS cells - confocal Live cell TauSTED on U2OS cells - sted

Live cell TauSTED on U2OS cells, using labels for actin (SiR-actin, glow), microtubules (SPY555-tubulin, cyan), and membranes (CF488A coupled to WGA, green). Scale bar: 10

STED for malaria research

STED for malaria research
STED for malaria research: STED nanoscopy of merozoites invading erythrocytes. 3D STED image showing overlays of RON4 (magenta) with the proteins PfRh5 (left, green), PfRipr (middle, green), PfCyRPA (right, green). Nuclear staining with DAPI is shown in blue, and erythrocytic membrane is shown in gray. Scale bar: 1 µm. Image courtesy: Jennifer Volz, Alan Cowman, Walter and Eliza Hall Institute of Medical Research, Australia, and Marko Lampe, EMBL, Germany.

Three color STED imaging of Cos 7 cells

Three color STED imaging of Cos 7 cells
Three color STED imaging of Cos 7 cells stained with antibodies against NUP153 (green), TOM20 (magenta), and with SiR–actin (gray). Confocal (top) and STED (bottom). Scale bar: 2μm. Sample courtesy: Urs Ziegler and Jana Doehner, ZMB, University of Zurich, Switzerland.

3D STED 775 deep nanoscopy of glomerulus in cleared kidney tissue

3D STED 775 deep nanoscopy of glomerulus in cleared kidney tissue immunostained for nephrin.
3D STED 775 deep nanoscopy of glomerulus in cleared kidney tissue immunostained for nephrin. Scale bar: 10 μm, z-depth color coded. Image taken with STELLARIS confocal platform. Sample courtesy: David Unnersjö Jess, KTH, Stockholm, Sweden.

STED and DNA origami imaging

STED and DNA origami imaging: TauSTED 775 delivers resolutions <30nm on GATTA-Bead R whose nominal size is 23 nm. Scale bar: 1 µm.

TauSTED 660 at extremely low STED power on COS7 cells immunostained for nuclear pores (NUPs) with AF555

TauSTED 660 at extremely low STED power on COS7 cells immunostained for nuclear pores (NUPs) with AF555. The primary antibody recognizes several proteins of the nuclear pore basket, so the NUPs are labeled in the central region and as a result they appear as point-like structures.

STED for Microbiology

Image acquired with STELLARIS confocal platform.

STED for Microbiology
STED for Microbiology: Bacterial flagella visualized with correlative three-color confocal-LIGHTNING-STED imaging which enables the investigation and validation of samples using complementary approaches. Sample courtesy of Marc Erhardt, Humboldt-Universität, Berlin, Germany.

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