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Fluorescence Microscopy

Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy, mainly because of its high sensitivity and high specificity. Fluorescence is a form of luminescence. Fluorescence microscopy even allows users to determine the distribution of a single molecule species, its amount and its localization inside a cell. Colocalization and interaction studies can be performed, and ion concentrations as well as intra- and intercellular processes like endocytosis and exocytosis can be observed. With the help of super-resolution fluorescence microscopy, it is even possible to image sub-resolution structures. Read more about fluorescence microscopy, quantitative fluorescence and super-resolution on Leica Science Lab.

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Our experts on solutions for fluorescence applications are happy to help you with their advice.

Fluorescence Microscopes & Imaging Products 29

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THUNDER Imager Model Organism

The THUNDER Imager Model Organism allows fast and easy 3D exploration of whole organisms for developmental or molecular biology research.

THUNDER Imager 3D Live Cell & 3D Cell Culture & 3D Assay

THUNDER Imagers provide you with a solution for advanced 3D cell culture assays, whether you want to study stem cells, spheroids, or organoids.

THUNDER Imager Tissue

The THUNDER Imager Tissue allows real-time fluorescence imaging of 3D tissue sections typically used in neuroscience and histology research.

High Speed High Resolution Monochrome Fluorescence sCMOS Camera Leica DFC9000

Leica DFC9000

4.2 MP cooled monochrome fluorescence scmos camera for advanced applications like high-speed live cell imaging, FRAP, and ratio measurement

2.8 MP Color Microscope Camera Leica DFC7000 T

DFC7000 T and DFC7000 GT

Cooled 2.8 megapixel digital microscope cameras

Software platform for life science applications

LAS X Life Science

Software platform for life science applications

Leica M205 FCA & Leica M205 FA

(Semi)-automated fluorescence stereo microscopes

Leica DM4 B & DM6 B

Increase work efficiency with Leica DM4 B & DM6 B upright digital research microscopes.

Ergonomic microscope system Leica DM2500 LED for demanding tasks in life science applications

Leica DM2500 & DM2500 LED

Ergonomic optical microscope systems for routine and research applications

The Leica DM1000 LED combines the advantages of a fully ergonomic system microscope with innovative LED illumination, and unlimited possibilities in mobile use.

Leica DM1000 LED

Uniquely ergonomic system microscope with LED illumination

Fluorescence Microscope Camera Leica DFC3000 G

Leica DFC3000 G

1.3 MP monochrome camera for basic fluorescence applications such as documentation of fixed, immunostained samples

Inverted Laboratory Microscope with LED Illumination

Leica DM IL LED

Inverted Laboratory Microscope with LED Illumination

Uniquely Ergonomic System Microscope Leica DM1000

Leica DM1000

Uniquely Ergonomic System Microscope

Leica DM6 FS fixed stage fluorescence microscope

Leica DM6 FS

Fixed Stage Microscope for Electrophysiology and in vivo Imaging

Leica TL4000 BFDF

Transmitted Light Base For Brightfield and Darkfield Applications Absorbs Shock and Vibrations for High Quality Imaging

Simultaneous use of 1X stereo objective and 10X or 20X compound objective for high resolution imaging.

Leica FluoCombi III

Fluorescence stereomicroscope accessory

LED Multi Contrast Illumination LED3000 MCI

Leica LED3000 MCI

LED Multi Contrast Illumination for Routine Stereo Microscopes

The Leica LAS X Widefield System performs workflow-guided advanced image analysis and includes a full range of image enhancement tools.

LAS X Widefield Systems

Fluorescence Microscope System for Advanced Imaging and Analysis

The uniquely ergonomic system microscope Leica DM3000 boosts cytology and pathology workflows thanks to its intelligent automation.

Leica DM3000 & DM3000 LED

Uniquely Ergonomic System Microscopes With Intelligent Automation

Fluorescence Light Microscopes

Fluorescence microscopes used in research applications are based on a set of optical filters:

  • an excitation filter
  • a dichroic beam splitter and
  • an emission filter

The filters are often plugged in together in a filter cube (compound microscopes) or in a flat holder (mainly stereo microscopes).

Whereas the excitation filter selects the wavelengths to excite a particular dye within the specimen, the emission filter serves as a kind of quality control by letting only the wavelengths of interest emitted by the fluorophore pass through. The dichroic mirror's purpose is to reflect light in the excitation band and transmit light in the emission band,  enabling the classic epifluorescence incident light illumination.

This fluorescence tutorial explains the optical elements in the light path and the operating mode of fluorescence microscopy taking the example of an inverted microscope which can be used for transmitted light contrasting methods and fluorescence microscopy.

Fluorescence Stereo Microscopes

Leica Microsystems’ fluorescence stereo microscopes use the TripleBeam technology, a separate (third) beam path for fluorescence illumination of the sample without a dichroic mirror.

Therefore one excitation filter for the illumination beam path and two emission filters, one for each observation beam path, are needed.

Cameras for fluorescence microscopy

To acquire fluorescence images, it is necessary to use the appropriate CCD or sCMOS fluorescence camera. Leica Microsystems offers dedicated fluorescence cameras for all application needs. Each camera provides the perfect match of sensitivity and image quality to ensure superb results.

Software for fluorescence microscopy

Straightforward setup and control of experiments is becoming more and more important In fluorescence microscopy. The dedicated Leica Application Suite (LAS) X software platform facilitates the setup of even complex experiments. LAS X guides you from the setup to the analysis of the imaging data. From easy multi-channel experiments to complex 3D analysis, LAS X is the perfect software platform.

News

Image Gallery: THUNDER Imager

To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using…

Leica Science Lab

Development of fluorescence lifetime imaging microscopy (FLIM) and its relevance for functional imaging

Prof. Ammasi Periasamy, Director, Keck Center for Cellular Imaging, University of Virginia, was interviewed by Dr. Giulia Ossato, Product Manager…

Leica Science Lab
Human Kidney Organoid, hESC-derived Podocytes

Multiphoton imaging bolsters findings on in vitro differentiation of pluripotent stem cell-derived podocyte

The renal corpuscle of the kidney comprises a glomerular vasculature embraced by podocytes and supported by mesangial myofibroblasts. Their combined…

Leica Science Lab

Cellular Motility: Microtubules, Motor Proteins and Tau-Proteins

Cellular motility is based on motor-proteins that can bind to filamentous scaffold proteins and – under consumption of ATP – can “crawl” on these…

Leica Science Lab

How FRET/FLIM Biosensors and Lifetime Imaging Helped Identify the Importance of Zinc for Human B-Cell Development

In her latest studies, Dr. Mukta Deobagkar-Lele investigated the role of ZIP7, a zinc transporter protein, in modulating B cell activity and its…

Leica Science Lab
Cavanillesia Arborea 10mm Core

Improved tree-ring visualization using autofluorescence

Autofluorescence can be a very practical tool for the visualization of growth rings in wood, especially when these structures are hard to distinguish,…

Leica Science Lab

Single-residue bioorthogonal labeling of G protein-coupled receptors (GPCRs) in live cells for quantitative analysis

To perform quantitative studies of membrane bound G protein-coupled receptors (GPCRs) with high-performance microscopy, the proteins must first be…

Leica Science Lab
Drosophila melanogaster fruitflies perching on the brim of a bowl of sour dough and waiting for their chance. Image Courtesy: Ursula Gönner, Dolgesheim (Germany).

Visualize developmental patterns in Drosophila

The marvelous complexity of life is perhaps the greatest source of fascination within the biological sciences. Among the most complex subjects within…

Leica Science Lab

Phasor Analysis for FLIM (Fluorescence Lifetime Imaging Microscopy)

The Phasor analysis approach to analyze fluorescence lifetime does not require any fitting. Phasor FLIM (fluorescence lifetime imaging microscopy)…

Leica Science Lab

Visualize mechanical interactions of Cancer Cells

Cells interact with their environment. Not only on the base of chemical signals, but also by sensing and modifying mechanical properties of the…

Leica Science Lab