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  • Live Cell Imaging

    Shifting perspective from single microscope components to a full working live cell imaging solution, Leica Microsystems integrates microscope, LAS X imaging software, cameras, and dedicated…
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  • Leica photomanipulation

    Photomanipulation

    The term photomanipulation encompasses a range of techniques that utilize the properties of fluorescent molecules to initiate events and observe how dynamic complexes behave over time in living cells.…
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  • Science Lab Topic: TIRF Microscopy

    TIRF Publication List

    This monthly updated references list presents current papers using Leica AM TIRF in the major application fields for TIRF microscopy.
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  • Science Lab Topic: Live-Cell Imaging

    Where to go? Cellular Migration requires coordinated Transitions of Actin Cortex

    Plants, Bacteria, and Fungi possess a rigid cell wall that protects the cell and gives it shape. Animal cells, such as mammalian cells, have no outer wall, which exposes their plasma membrane to the…
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  • Science Lab Topic: Super-Resolution

    The Molecular Architecture of Hemidesmosomes as Revealed by Super-Resolution Microscopy

    Hemidesmosomes have been extensively studied by immunofluorescence microscopy, but due to its limited resolution, their precise organization remained poorly understood. We studied hemidesmosome…
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  • Science Lab Topic: Super-Resolution

    Initiation of Lamellipodia and Ruffles Involves Cooperation Between mDia1 and the Arp2/3 Complex

    Protrusion of lamellipodia and ruffles requires polymerization of branched actin filaments by the Arp2/3 complex. Although regulation of Arp2/3 complex activity has been extensively investigated, the…
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  • Science Lab Topic: Super-Resolution

    Botulinum Neurotoxin Type-A Enters a Non-Recycling Pool of Synaptic Vesicles

    Neuronal communication relies on synaptic vesicles undergoing regulated exocytosis and recycling for multiple rounds of fusion. Whether all synaptic vesicles have identical protein content has been…
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  • Science Lab Topic: TIRF Microscopy

    Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy

    Total Internal Reflection Fluorescence (TIRF) Microscopy is a technique that only illuminates dye molecules near a surface. In this video, the pioneer of TIRF Microscopy describes what this technique…
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  • Science Lab Topic: Super-Resolution

    Finding, Defining and Breaking the Diffraction Barrier in Microscopy – A Historical Perspective

    Diffraction plays a crucial role in microscopy as it prevents the recording of arbitrarily sharp images with conventional light microscopes. Many names are connected with the notion of diffraction and…
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  • Science Lab Topic: TIRF Microscopy

    Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results

    The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the…
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  • Science Lab Topic: TIRF Microscopy

    Total Internal Reflection Fluorescence (TIRF) Microscopy

    Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy…
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  • Science Lab Topic: TIRF Microscopy

    Protein Transport Processes at the Apical Membrane of Polarized Epithelial Cells

    Due to their special role in organ function and the exchange of biological components some body cells developed certain polarization characteristics. These are reflected in differences of their plasma…
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  • Science Lab Topic: Live-Cell Imaging

    TIRF Microscopy of the Apical Membrane of Polarized Epithelial Cells

    Application of TIRF microscopy (Total Internal Reflection Fluorescence) allows the visualization of structures at the apical surface of polarized epithelial cells that have been hidden in conventional…
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  • Science Lab Topic: Live-Cell Imaging

    Exploring Cell Logistics

    Using TIRF microscopy, scientists have been able to take a closer look at intracellular transport processes with the example of the galactose-binding protein Galectin-3, which has been identified as a…
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