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DMi8 S module Infinity TIRF
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Multicellular Organization during Stem Cell Dynamics
Multicellular organization, e.g. during tissue morphogenesis or of human embryonic stem cells, relies on collective cytoskeletal dynamics. During their studies of multicellular developing human…
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Phosphoprotein Regulates Integrin Endosomal Trafficking
Integrins are involved in cell-cell and cell-extracellular matrix adhesion. In addition, they mediate several signaling pathways, regulating e.g. cell motility. Caliva et al. discovered, with the help…
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Studying Actin Cytoskeleton and Microfilament Assembly
This article demonstrates how actin microfilament assemblies can be clearly visualized with superior resolution at the plasma membrane of a cell using a DMi8 S Infinity TIRF High Power imaging…
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Plant Cell Development and Morphogenesis
The article discusses how tubulin molecules, which make up microtubules, in plant cells can be studied with total internal reflection fluorescence (TIRF) microscopy to better understand tubulin…
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Where to go? Cellular Migration requires coordinated Transitions of Actin Cortex
Plants, Bacteria, and Fungi possess a rigid cell wall that protects the cell and gives it shape. Animal cells, such as mammalian cells, have no outer wall, which exposes their plasma membrane to the…
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Initiation of Lamellipodia and Ruffles Involves Cooperation Between mDia1 and the Arp2/3 Complex
Protrusion of lamellipodia and ruffles requires polymerization of branched actin filaments by the Arp2/3 complex. Although regulation of Arp2/3 complex activity has been extensively investigated, the…
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Botulinum Neurotoxin Type-A Enters a Non-Recycling Pool of Synaptic Vesicles
Neuronal communication relies on synaptic vesicles undergoing regulated exocytosis and recycling for multiple rounds of fusion. Whether all synaptic vesicles have identical protein content has been…
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Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy
Total Internal Reflection Fluorescence (TIRF) Microscopy is a technique that only illuminates dye molecules near a surface. In this video, the pioneer of TIRF Microscopy describes what this technique…
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Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results
The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the…
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Total Internal Reflection Fluorescence (TIRF) Microscopy
Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy…
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TIRF Microscopy of the Apical Membrane of Polarized Epithelial Cells
Application of TIRF microscopy (Total Internal Reflection Fluorescence) allows the visualization of structures at the apical surface of polarized epithelial cells that have been hidden in conventional…
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Exploring Cell Logistics
Using TIRF microscopy, scientists have been able to take a closer look at intracellular transport processes with the example of the galactose-binding protein Galectin-3, which has been identified as a…
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Fields of Application
Live Cell Imaging
Shifting perspective from single microscope components to a full working live cell imaging solution, Leica Microsystems integrates microscope, LAS X imaging software, cameras, and dedicated…
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Photomanipulation
The term photomanipulation encompasses a range of techniques that utilize the properties of fluorescent molecules to initiate events and observe how dynamic complexes behave over time in living cells.…
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Super-Resolution
Find out more about Leica super-resolution microscopy solutions and how they can empower you to visualize in fine detail subcellular structures and dynamics.
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Cell Biology
When your research is centered on understanding the cellular basis of human health and disease, it is critical to investigate the cells of interest in spatiotemporal and molecular detail.…
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Virology
Do your research interests focus on viral infection and disease? Find out how you can gain insights into virology with solutions for imaging and sample preparation from Leica Microsystems.
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